FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760401
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT12810216.84663749784653No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11940015.702241317410156No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT8055510.593752506900964No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG683568.989467399437927No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA623698.202119671068292No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCATCGTTGAAAATTT511386.725135816496822No Hit
GCTGCGTTCTTCATCGATGCACTTGCCGAGGGATCCTCCATTGCACGTCT391355.146626582553153No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAGAGTTG345564.5444443129348855No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT152942.011307191863241No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT70050.9212244591998169No Hit
GCTGCGTTCTTCATCGATGCCGTGAGCCCGGGAGATTCGATGCCGAACAG43500.5720665806594152No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT39950.5253806872952561No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG33740.4437132512976706No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCATCGTTGAAAATTT29650.38992584175980827No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA29010.38150922999838244No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCGAGATATCCATCGTTGAAAATTT15600.20515491168475583No Hit
GCTGCGTTCTTCATCGGTATCGGAGCCTAGATATCCATCGTTGAAAATTT11410.15005240655917074No Hit
GCTGCGTTCTTCATCGGTCTAGGAGCCTAGATATCCATCGTTGAAAATTT10750.1413727756802003No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTCGAAAGTTT10450.13742748891703194No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGCCG10230.1345342786240418No Hit
GCTGCGTTCTTCATCGATGCACTGGCCGAGGGATCCTCCATTGCACGTCT10000.13150955877227938No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG8790.11559690216083356No Hit
GCTGCGTTCTTCATCGATATAGGAGCCTAGATATCCATCGTTGAAAATTT7640.10047330290202143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)