FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757500
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGACCCTCTGGG12292216.227326732673266No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCA10558813.939009900990099No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA7770410.257953795379537No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGT600277.924356435643565No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG530337.0010561056105605No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAAGTTTCGGGCCTGCC477286.300726072607262No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG411235.428778877887789No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGCCTTCGGGCTAA363414.797491749174918No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAATGAGAACGTGCCCTCACGG240113.1697689768976898No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC138631.83009900990099No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGTGACCCCGGTCTAACC106601.407260726072607No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA26340.3477227722772277No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG24660.32554455445544556No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCGGACCCTCTGGG18360.24237623762376237No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATGCTTAGGGGAAATC13700.18085808580858084No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGCGGACCCTCTGGG13050.1722772277227723No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACAAGTGACCCCGGTTTACCA10540.13914191419141914No Hit
TCCGTAGGTGAACCTGCGGACCCCGGCCCCAGCCAAACCAAGGACACGAC9700.12805280528052804No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCTGACCCTCTGGG8970.11841584158415841No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGCGCGT8430.11128712871287127No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGCAAGG7800.10297029702970298No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGACCCTCCGGG7700.10165016501650166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)