FastQCFastQC Report
Wed 8 May 2024
EGAF00002462853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744266
Total Bases179.9 Mbp
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA12870017.2922046687609No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA8050310.816428534959275No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAAATTGTGAGGGAGAGTCT7726010.380697223842013No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA485816.527370590622169No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAAACAATGCACTTATAAC479766.446082449016884No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATCACACCCTTCAAAAACATG400555.381812416528499No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAATTCAACTC304834.095713091824697No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC271693.65044218061822No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG245113.293311799813507No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAATATAGACCGTCGTGA239943.223847387896263No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACACGTACTTAACAGTACTT138391.859415854009185No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTAATACACCTTAA137421.8463828792394117No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG87451.174983137749138No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC58320.7835908129620324No Hit
TCCGTAGGTGAACCTGCAGGTGCAAAGGCCCGGGCAGGTGGAAGTGGTGC26190.35189031878387567No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA24090.32367460021013994No Hit
TCCGTAGGTGAACCTGTGGAAGGATCATTATCACACCCTTCAAAAACATG14750.1981818328393343No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA13800.1854175791988348No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAAATTGTGAGGGAGCGTCT13230.17775902701453514No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA12790.17184716217051432No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACAAACAATGCACTTATAAC10620.1426909196443207No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA8530.11460956163522182No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAAATTGTGAGGGCGAGTCT8180.10990694187293254No Hit
TCCGTAGGTGAACCTGCGGGTGCAAAGGCCCGGGCAGGTGGAAGTGGTGC7600.10211402912399599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)