FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002462854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744266
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT13217717.75937635200318No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11302215.185699736384572No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCCAGAGATCCATTGTTCACAGTTG8701111.69084708961581No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG499966.7174907895832945No Hit
GCTGCGTTCTTCATCGTTATGGGAGCCAAGACATCCATCATTGAAAATTT494636.645876608631861No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAGAATTT296683.986209231645675No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA254293.416654798150124No Hit
GCTGCGTTCTTCATCGTTGTGGGAGCCAAGACATCCGTTGCTGAATGTGT249143.347459107362153No Hit
GCTGCGTTCTTCATCGATGCACGAGCCAAGAGATCCACCGTTAGAAGTTG248753.3422190453413156No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCAAGACATCCGTTGCTGAATGTGT178702.401023290060274No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATCCGTTGCTAAGAGTTG145601.9562898211123443No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT142491.9145036855102882No Hit
GCTGCGTTCTTCATCGATGCATAAGCCGTGTGCTCCATTGCACAGAGTTT60670.8151655456516891No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG44720.6008604450559343No Hit
GCTGCGTTCTTCCACACTGCTCCCAGCCCTGGCTTCTCGCCTGTAAACTG35690.4795328551888706No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG33130.44513655064184043No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA22660.30446103946707226No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT16440.22088876826295975No Hit
GCTGCGTTCTTCATCGATATGGGAGCCAAGACATCCATCATTGAAAATTT14380.19321049194777137No Hit
GCTGCGTTCTTCATCGGTATGGGAGCCAAGACATCCATCATTGAAAATTT10690.14363144359677857No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG10340.1389288238344893No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT9100.12226811381952152No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG7990.10735409114483263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)