FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667716
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA11366117.02235681038046No Hit
GCTGCGTTCTTCATCGTTATGGGAGCCTAGACATCCATCGTTGAAAATTT10783116.149231110232495No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCATCGTTGAAAATTT8045212.048835133499871No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7600411.382683655925572No Hit
GCTGCGTTCTTCATCGATGTGGGAGCCTAGACATCCGTTGCTGAAAGTTG376775.642668439875636No Hit
GCTGCGTTCTTCATCGATGCCAGAACAAAGAGATCCGTTGTTGAAAGTTT274944.117618867901922No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT263853.9515302913214603No Hit
GCTGCGTTCTTCATCGTTGTGGGAGCCTAGACATCCGTTGCTGAAAGTTG192792.887305381329787No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGATATCCATCGTTGAAAATTT182882.7388889887317363No Hit
GCTGCGTTCTTCATCGTTACGAAAGCCGAGATATCCATCGCTGAGGTTTT155912.3349747497439033No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT113851.7050662257606528No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG85491.2803347530986227No Hit
GCTGCGTTCTTCATCGATATGGGAGCCTAGACATCCATCGTTGAAAATTT35510.5318129264537618No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA29490.4416548352892547No Hit
GCTGCGTTCTTCATCGCCTCCTAATGCCAAGGCATCCACCGAACGCCCTT28610.42847557943796466No Hit
GCTGCGTTCTTCATCGATGCGGGAGCCTAGACATCCGTTGCTGAAAGTTG18620.27886107267161486No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGATATCCATCGTTGAAAATTT13790.2065249297605569No Hit
GCTGCGTTCTTCATCGGTATGGGAGCCTAGACATCCATCGTTGAAAATTT12960.19409449526445374No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAAAGTTA9820.14706851415871416No Hit
GCTGCGTTCTTCATCGTTCTGGGAGCCTAGACATCCATCGTTGAAAATTT9550.14302487884070475No Hit
GCTGCGTTCTTCATCGGTATGGGAGCCGAGACATCCATCGTTGAAAATTT9150.13703430799920924No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGAGAGTTA8180.12250717370858269No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT7970.11936212401679755No Hit
GCTGCGTTCTTCATCGATACGAAAGCCGAGATATCCATCGCTGAGGTTTT7350.11007673921247957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)