FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002462857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002462857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801878
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACTC12431615.503106457590807No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG9517711.86926190767174No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA775049.665310683171255No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA389984.863333325019516No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC375364.68101132591242No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGACACCCGATCCATACCAACC374964.676023035923171No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAACGTATCCACGTATAAAC351744.386452802047194No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACATTGGCCTCTTGGCCC302013.766283649133659No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG299553.7356056656997696No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAAATTGAACTTAATAA185292.31070063027044No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATAAAACAATGAACTTATAA164982.0574202060662596No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTATGGTAAAAAGGTGAAAACC149131.8597592152422189No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTGGCGGCCCTCTGTGG142941.7825654276585716No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAACGTATCCAACGTATAAA104811.307056684433293No Hit
TCCGTAGGTGAACCTGCGTAGGGATCATTTCTGGTAAAAAGGTGAAAACC101681.2680233152674099No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC66560.8300514542112392No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACGTATCCACGTTATCAA66160.8250631642219888No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG63200.7881498183015371No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC45110.5625544035376953No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC20570.25652281269719335No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAACACTAATCCAAACCTACTC12670.1580040854095012No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTACCGAGTGAGGGCCCTCTGGG10240.12770022372480602No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGG10220.12745080922534352No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACCC9460.11797305824576805No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC8710.10862001451592386No Hit
TCCGTAGGTGAACCTGCAGAGGGATCATTACCGAGTTTACAACTCCCAAA8360.1042552607753299No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA8150.10163640853097355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)