FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002463500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316221
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG67032.1197200691921156No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG56461.7854601686794993No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG53911.704820362973996No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA44301.4009189775505106No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG43421.3730903387188074No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG37891.1982126424241275No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36161.1435040683572566No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG31340.9910790238472461No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG24770.7833129362060077No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG24430.7725609621119407No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA23810.752954421116877No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG23720.7501083103272711No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20240.6400586931291723No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA20040.6337340024856034No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG19890.6289904845029268No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19880.6286742499707484No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA18060.5711195651142713No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17260.5458208025399958No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG16050.5075564241464039No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG16040.5072401896142255No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA15580.492693401134017No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA15230.48162519250777147No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14910.4715056874780612No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA14600.4617024169805295No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG14490.45822383712656656No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG13780.43577118534189696No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA13420.42438674218347294No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12800.4047802011884094No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG12710.40193409039880335No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12500.395293165223056No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA12230.386754832854238No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG12200.3858061292577027No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11480.36303724294085465No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG11310.3576612558938211No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11200.3541826760398582No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11060.34975539258935995No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10480.33141378972301017No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG10300.32572156814379816No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA10040.31749947030715864No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG10010.31655076671062327No Hit
GACTACTGGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG9980.31560206311408795No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9940.3143371249853742No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9800.3099098415348759No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC9430.29820916384427343No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9280.2934656458615968No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9170.2899870660076339No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9110.2880896588145632No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8770.27733768472049614No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8580.2713292286091057No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8540.2700642904803919No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG8400.2656370070298936No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8260.2612097235793954No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8140.2574149091932541No Hit
GACTACAAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG8130.2570986746610756No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8060.25488503293582654No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8030.25393632933929117No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG7990.2526713912105774No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7960.25172268761404204No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG7450.23559472647294138No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7300.23085120849026475No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7270.2299025048937294No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC7220.22832133223283715No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7140.22579145597540962No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG7060.22326157971798202No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7040.22262911065362517No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7020.22199664158926827No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG6840.21630442001005626No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6840.21630442001005626No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6650.2102959638986658No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6590.20839855670559512No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA6520.20618491498034602No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6420.20302256965856158No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA6400.2023901005942047No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6390.20207386606202624No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6330.2001764588689556No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6180.1954329408862789No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG6160.194800471821922No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6110.19321929916102978No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6110.19321929916102978No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5880.18594590492092555No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5730.1812023869382489No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5610.17740757255210754No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5570.17614263442339378No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5560.17582639989121532No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG5410.17108288190853865No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG5400.1707666473763602No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5290.1672880675223973No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5170.16349325313625598No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5060.16001467328229307No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5050.15969843875011464No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4990.15780103155704397No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4820.1524250445100104No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4740.14989516825258284No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4680.14799776105951218No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4680.14799776105951218No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4670.14768152652733374No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4650.14704905746297683No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4540.14357047760901395No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4520.14293800854465707No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4470.14135683588376485No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4420.13977566322287263No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4420.13977566322287263No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG4370.13819449056198038No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4360.13787825602980194No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4270.13503214524019594No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4170.1318697999184115No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4080.1290236891288055No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4060.12839122006444859No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG3930.12428017114612881No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3920.12396393661395037No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG3910.12364770208177192No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3900.1233314675495935No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3880.12269899848523658No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3840.12143406035652281No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3820.12080159129216593No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3810.12048535675998748No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG3810.12048535675998748No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3800.12016912222780904No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3770.11922041863127371No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3750.11858794956691682No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3750.11858794956691682No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG3740.11827171503473836No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG3730.11795548050255991No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG3670.11605807330948925No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3600.11384443158424015No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3600.11384443158424015No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3520.11131455532681259No Hit
GANTANTGGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG3480.11004961719809879No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3450.10910091360156347No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3450.10910091360156347No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3440.10878467906938502No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3440.10878467906938502No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3440.10878467906938502No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG3430.10846844453720657No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG3410.10783597547284968No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3390.1072035064084928No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3260.10309245749017301No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3250.10277622295799457No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3250.10277622295799457No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3250.10277622295799457No Hit
GACTACTAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG3230.10214375389363767No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3210.10151128482928079No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3190.1008788157649239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)