FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002463502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371841
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA107432.8891380993489153No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA88272.3738640978267593No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG62121.670606522680393No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG53791.4465860408077647No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG51791.3927996105862452No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG51721.390917085528492No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG50571.3599898881511185No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA43861.1795364147579206No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG43851.1792674826068132No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG40341.0848722975680465No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG40321.0843344332658313No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA36490.9813334193916217No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA36420.9794508943338686No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA35570.9565916614897227No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA28840.7756003237943099No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA25690.6908866961954169No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA23040.6196196761519036No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG19570.5263002197175675No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG19540.5254934232642446No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA19400.5217283731487383No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG18820.5061303083844977No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA18700.5029031225712065No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG18510.49779341170016217No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA18380.49429729373576337No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA18260.49107010792247224No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA18060.48569146490032034No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17870.480581754029276No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG17690.47574097530933923No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG17240.46363902850949734No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17130.4606807748473138No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG15860.42652639165664896No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG15570.4187273592745286No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA14830.3988263800925664No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14690.3950613299770601No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA14540.39102734771044617No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA14180.38134579027057264No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG14000.3765050115506359No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG13930.3746224864928827No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG13700.368437047017408No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13570.36494092905300923No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG12870.3461156784754774No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12670.34073703545332545No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG12510.33643412103560394No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA12420.33401373167563553No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG12360.33240013876899No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12020.32325644563133166No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA11760.31626420970253416No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA11660.3135748881914582No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11610.3122302274359202No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10990.2955564340672492No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG10190.2740418619786414No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG10130.27242826907199585No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA10100.27162147261867303No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10030.2697389475609198No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG9990.2686632189564895No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9850.2648981688409831No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9250.2487622397745273No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9120.24526612181012852No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9070.24392146105459053No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8750.23531563221914745No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8660.23289524285917906No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8560.2302059213481031No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8440.2269787355348119No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8430.22670980338370433No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8280.2226758211170904No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA8270.22240688896598276No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8180.21998649960601438No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG8150.2191797031526916No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8130.21864183885047642No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8110.21810397454826125No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7630.20519523129509656No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA7560.20331270623734338No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG7510.2019680454818054No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7300.19632047030854585No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7150.1922864880419319No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA7040.18932823437974833No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6960.18717677717088754No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG6920.18610104856645718No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6910.18583211641534958No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6870.1847563878109192No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG6870.1847563878109192No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC6860.1844874556598116No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6630.17830201618433686No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6540.17588162682436848No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6530.17561269467326088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6270.16862045874446338No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6150.1653932729311722No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6040.16243501926898862No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5850.15732530839794429No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5810.1562495797935139No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5810.1562495797935139No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5740.1543670547357607No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG5740.1543670547357607No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5440.14629909020253282No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5420.14576122590031762No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGAA5320.14307190438924164No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5250.14118937933148845No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5250.14118937933148845No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATGAA5180.1393068542737353No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA5160.1387689899715201No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5140.1382311256693049No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA5110.13742432921598208No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5090.1368864649137669No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5090.1368864649137669No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATGAA5050.13581073630933652No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4970.13365927910047576No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4920.13231461834493774No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4870.13096995758939978No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4790.128818500380539No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC4770.1282806360783238No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4750.1277427717761086No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4700.12639811102057064No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4650.12505345026503262No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATGAA4570.12290199305617186No Hit
GANTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGAG4550.12236412875395668No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4460.1199437393939883No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4430.1191369429406655No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4390.1180612143362351No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGNA4380.11779228218512751No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4340.11671655358069713No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4330.11644762142958953No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4320.11617868927848193No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATGAA4300.11564082497626674No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATGNA4280.11510296067405155No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4270.11483402852294396No Hit
GANTANACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4260.11456509637183636No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4220.11348936776740595No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA4220.11348936776740595No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4200.11295150346519077No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4170.11214470701186798No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4120.11080004625633No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA4060.10918645334968441No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4010.10784179259414643No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3950.10622819968750083No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATGAA3880.10434567462974766No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGAA3880.10434567462974766No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3830.10300101387420968No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATGAA3810.10246314957199447No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATGAA3800.10219421742088687No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3750.1008495566653489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)