FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002463505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414100
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA355668.588746679546004No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA205134.953634387829027No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT193844.6809949287611685No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT109582.646220719632939No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA106722.57715527650326No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA95922.3163487080415357No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA93002.245834339531514No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC67911.6399420429847864No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC67061.6194156000965951No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT56981.3759961361989859No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT54031.3047573049987926No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA52661.271673508814296No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA50771.2260323593334943No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT49621.1982612895435885No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT45431.097078000482975No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA43841.058681477903888No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA40520.9785076068582468No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA38230.9232069548418256No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC38130.9207920792079207No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC37930.915962327940111No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT36070.8710456411494809No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT28960.6993479835788458No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA27830.6720598889157209No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT27230.6575706351122917No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT27190.6566046848587298No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT26020.628350639942043No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA25400.6133784110118329No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT24850.6000965950253562No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT24850.6000965950253562No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23060.5568703211784592No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA22430.5416566046848587No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA22110.5339290026563632No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21230.5126780970780005No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA20550.4962569427674475No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT20200.4878048780487805No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC19770.4774209128229896No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA19720.47621347500603717No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA19290.4658295097802463No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC19250.46486355952668434No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT17810.43008935039845453No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC16830.40642356918618694No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC16790.405457618932625No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC16310.3938662158898817No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC15990.38613861386138615No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15830.3822748128471384No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC15270.3687515092972712No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15140.3656121709731949No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT13790.33301134991547937No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA13660.32987201159140306No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT13310.32141994687273606No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT13070.3156242453513644No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12980.31345085728085004No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA12740.3076551557594784No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT12610.30451581743540207No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA12450.3006520164211543No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC12040.2907510263221444No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA11660.28157449891330594No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA11370.27457135957498185No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA11290.27263945906785797No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA11290.27263945906785797No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT11220.27094904612412457No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11120.26853417049021977No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA10920.26370441922241006No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10750.2595991306447718No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA10650.25718425501086695No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA10540.2545278918135716No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC10540.2545278918135716No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT10500.25356194156000966No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT10040.24245351364404732No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT9880.23858971262979956No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9860.23810673750301858No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT9820.23714078724945664No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9620.23231103598164693No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9610.23206954841825644No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC9310.22482492151654188No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC9180.22168558319246556No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA9100.21975368268534168No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9050.2185462448683893No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9030.21806326974160833No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA8770.2117845930934557No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8660.20912822989616037No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC8400.20284955324800774No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC8150.1968123641632456No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8120.19608790147307414No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7870.190050712388312No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7860.18980922482492152No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC7760.18739434919101666No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC7670.1852209611205023No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7460.18014972228930212No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC7010.16928278193673027No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6970.16831683168316833No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6900.16662641873943493No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6710.1620381550350157No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA6700.16179666747162522No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA6650.1605892296546728No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT6600.15938179183772036No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6520.15744989133059648No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA6270.15141270224583434No Hit
CCTANGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5980.14440956290751025No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT5970.14416807534411977No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5870.1417531997102149No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT5820.14054576189326248No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA5810.140304274329872No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5750.13885534894952908No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5580.13475006037189083No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5550.1340255976817194No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA5530.13354262255493843No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA5510.13305964742815746No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5470.13209369717459551No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA5450.13161072204781454No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5400.13040328423086212No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA5390.13016179666747163No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC5380.12992030910408114No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAGACCCTGAT5240.12653948321661435No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA5060.1221927070755856No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5010.12098526925863318No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA5000.12074378169524269No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5000.12074378169524269No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA4970.12001931900507123No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC4960.11977783144168075No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC4960.11977783144168075No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4930.11905336875150929No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4920.1188118811881188No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC4880.11784593093455686No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4850.11712146824438541No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT4810.11615551799082346No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4660.11253320453996618No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGGGGGAACCCTGAC4580.1106013040328423No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT4550.10987684134267084No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT4540.10963535377928038No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA4530.1093938662158899No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC4370.10553006520164213No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4350.10504709007486115No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT4340.10480560251147067No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4230.10214923931417533No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC4200.10142477662400387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)