FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198979
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG51402.5831871705054303No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG45322.2776272873016747No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG42372.1293704360761687No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG36301.824313118469788No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18810.9453258886616176No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG18690.9392951014931225No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG18380.9237155679745099No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG16670.8377768508234537No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15600.7840023319043719No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG14820.7448022153091532No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA12480.6272018655234974No Hit
GANTANTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG12450.6256941687313736No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11990.6025761512521423No Hit
GANTANAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG11420.5739299122017901No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA10790.5422682795671905No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10040.5045758597640957No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10030.5040732941667211No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG9950.5000527693877244No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9280.4663808743636263No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9130.45884239040300734No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9110.45783725920825813No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8920.44828851285814086No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8640.4342166761316521No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG8600.43220641374215374No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8170.410596093055046No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8050.4045653058865509No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7890.39652425632855726No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG7500.376924198030948No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG7280.3658677548887069No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG7230.36335492690183385No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA7230.36335492690183385No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7070.3553138773438403No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG6930.34827795898059594No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6850.34425743420159916No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG6850.34425743420159916No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6640.3337035566567326No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6610.3321958598646088No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6300.3166163263459963No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6210.3120932359696249No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG6000.3015393584247584No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5990.30103679282738377No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5710.28696495610089506No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5390.270882856984908No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5250.2638469386216636No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5180.2603289794400414No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4790.2407289211424321No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG4750.23871865875293372No Hit
GACTACTGGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG4740.23821609315555914No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTCTTTCAG4690.23570326516868614No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4600.23118017479231476No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4590.23067760919494015No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4570.22967247800019097No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4540.22816478120806719No Hit
GANTANTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG4540.22816478120806719No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4510.2266570844159434No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4450.22364169083169577No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG4430.22263655963694662No Hit
GACTACAAGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG4360.21911860045532444No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA4330.21761090366320063No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTCTTTCAG4300.21610320687107687No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA4130.20755959171570867No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4120.2070570261183341No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4070.2045441981314611No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3720.1869544022233502No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA3720.1869544022233502No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3490.17539539348373445No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3350.1683594751204901No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3340.1678569095231155No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG3330.16735434392574092No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3320.1668517783283663No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3310.1663492127309917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3290.1653440815362425No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3240.16283125354936953No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3130.15730303197824896No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3080.15479020399137597No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA3070.1542876383940014No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3000.1507696792123792No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC2990.1502671136150046No Hit
GANTANAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2970.1492619824202554No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2960.14875941682288082No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG2910.14624658883600783No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2860.14373376084913483No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2840.14272862965438562No Hit
GANTANTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2790.14021580166751266No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2790.14021580166751266No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2790.14021580166751266No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2730.13720040808326506No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2710.13619527688851588No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC2690.13519014569376667No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG2680.1346875800963921No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG2680.1346875800963921No Hit
GANTANACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG2670.13418501449901749No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2670.13418501449901749No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG2640.13267731770689367No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG2620.13167218651214452No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG2610.13116962091476989No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2610.13116962091476989No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2590.1301644897200207No Hit
GANTANTGGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG2550.1281542273305223No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2540.12765166173314774No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2540.12765166173314774No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG2530.1271490961357731No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG2520.12664653053839853No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG2520.12664653053839853No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2510.12614396494102392No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2490.12513883374627474No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG2470.12413370255152555No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG2470.12413370255152555No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2470.12413370255152555No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2460.12363113695415096No Hit
GANTANCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2450.12312857135677634No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2400.12061574336990336No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2400.12061574336990336No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2380.11961061217515415No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2330.11709778418828118No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2330.11709778418828118No Hit
GANTANAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG2330.11709778418828118No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG2320.11659521859090657No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2310.11609265299353198No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2300.11559008739615738No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2280.1145849562014082No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2270.11408239060403359No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2260.113579825006659No Hit
GANTANCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGAG2260.113579825006659No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2220.1115695626171606No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG2210.111066997019786No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2200.11056443142241142No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA2170.10905673463028762No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG2150.10805160343553843No Hit
GANTANTGGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG2120.10654390664341462No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2110.10604134104604003No Hit
GANTANAAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2110.10604134104604003No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA2080.10453364425391624No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2080.10453364425391624No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG2070.10403107865654165No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2070.10403107865654165No Hit
GANTANTGGGGTATCTAATNNTATTTGCTCNNNANGNTTTCGTGACTGAG2030.10202081626704326No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG2030.10202081626704326No Hit
GANTANTGGGGTATNTAATCNTATTTGCTCCCNACGCTTTCGTGACTGAG2020.10151825066966867No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG2020.10151825066966867No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2000.10051311947491946No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA1990.10001055387754486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)