FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297290
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA252988.509536143159877No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA211337.108547209795149No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA157545.2992027986141474No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT141944.774462645901308No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA132844.4683642234854855No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT83082.794577685088634No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA81532.7424400417101147No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC70542.372767331561775No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA69962.3532577617814256No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA69842.34922129906825No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA58101.9543206969625617No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT46351.559083722964109No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC45971.5463015910390527No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC43761.4719634027380673No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT40041.3468330586296209No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA38211.2852770022536917No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA34471.1594739143597161No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA33451.1251639812977228No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA28610.9623599851996367No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC27460.9236772175317031No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA25650.862793904941303No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC22450.7551548992566182No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT21360.7184903629452722No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC20650.6946079585589828No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT20280.6821621985266911No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT18770.6313700427192304No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA16300.5482861852063642No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA15390.5176763429647818No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC14810.4981667731844327No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT14430.4853846412593764No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14120.4749571125836725No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA13910.46789330283561503No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT13330.4483837330552659No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC13290.4470382454842073No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA12900.43391974166638636No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA12470.4194557502775068No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT12420.4177738908136836No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA11970.40263715563927477No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA11240.378082007467456No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10890.3663089912206936No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC10410.35016314036799084No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10350.348144909011403No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9690.32594436408893673No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9400.3161895791987621No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA9220.3101348851289986No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT8740.29398903427629586No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA8720.2933162904907666No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC8650.29096168724141414No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8540.2872615964210031No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8260.2778431834235931No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA8040.27044300178277103No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA8000.26909751421171246No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT7590.2553062666083622No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA7530.25328803525177435No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC7530.25328803525177435No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7210.24252413468330586No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT7110.23916041575565944No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC6920.2327693497931313No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6830.22974200275824955No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC6810.22906925897272024No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC6780.2280601432944263No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA6690.22503279625954456No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6480.2179689865114871No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6350.2135961519055468No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC6090.20485048269366612No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6010.202159507551549No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5830.1961048134817855No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT5730.19274109455413904No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC5560.18702277237714016No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA5380.18096807830737663No Hit
CCTACGGGAGGCAGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGAC5130.17255878098826063No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC5030.16919506206061422No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA4820.16213125231255676No Hit
CCTANGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4720.15876753338491034No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4480.15069460795855896No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4470.15035823606579435No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC4450.14968549228026506No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4450.14968549228026506No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA4370.14699451713814793No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4340.145985401459854No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4280.14396717010326615No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4170.14026707928285512No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4130.13892159171179655No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4090.137576104140738No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA4070.13690336035520873No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC4030.13555787278415016No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA4010.13488512899862087No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3980.13387601332032698No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3910.13152141007097445No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3900.13118503817820984No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC3760.12647583167950488No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3710.12479397221568166No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT3690.12412122843015237No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA3640.12243936896632918No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA3540.11907565003868277No Hit
CCTANGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3500.11773016246762422No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT3490.11739379057485956No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3480.11705741868209493No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA3410.11470281543274244No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3400.11436644353997781No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3320.11167546839786067No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3220.10831174947021427No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3180.10696626189915572No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3170.10662989000639106No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC3140.10562077432809715No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA3110.10461165864980321No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC3080.10360254297150931No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA3020.10158431161492146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)