FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002463514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309599
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG87222.8171925619914795No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG75182.4283024169974707No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA53371.7238427772699523No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA47271.5268137170985694No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG40221.2990998032939383No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA38751.2516190297772278No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34521.1149906814944492No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32811.0597579449546026No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG30350.9803003239674547No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG29480.9521994580085853No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG26310.8498089464113256No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG22040.7118886042913575No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21750.7025216489717344No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA21240.6860487275475696No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA19820.640182946327346No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA18770.6062681081011243No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18600.6007771342930694No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17830.5759062529271736No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA17770.5739682621713894No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA17770.5739682621713894No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA17130.5532963607763591No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA16260.5251954948174897No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG16130.5209965148466241No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG15240.49224965196916004No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG14570.47060875519623774No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14560.47028575673694034No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14530.4693167613590483No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA14400.46511778138818277No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13910.4492908568826126No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13820.44638387074893654No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13730.4434768846152604No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA13480.4354019231328266No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13380.43217193853985314No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12940.4179600063307698No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12500.40374807412168645No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG12480.4031020772030917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC11210.3620812728723284No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11200.36175827441303104No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10960.3540063113898947No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10610.34270136531448747No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC10400.33591839766924314No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG9860.3184764808671863No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9640.3113705147626446No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9540.3081405301696711No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG9320.3010345640651294No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9110.2942515964198851No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG8800.2842386441816672No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG8800.2842386441816672No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8640.2790706688329097No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8560.27648668115853087No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8320.2687347181353945No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG7860.25387678900771643No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7760.25064680441474296No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG7660.24741681982176944No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7620.24612482598458002No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7520.24289484139160658No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7450.24063385217652514No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA7410.23934185833933572No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7350.23740386758355161No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7110.22965190456041523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7100.22932890610111792No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7030.22706791688603645No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6980.2254529245895497No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6970.22512992613025234No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6880.22222293999657625No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6820.22028494924079212No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6750.21802396002571067No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6690.21608596926992657No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6660.21511697389203452No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6550.21156399083976368No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6350.20510402165381672No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6150.1986440524678697No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6130.19799805554927505No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6100.19702906017138297No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6050.19541406787489624No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG6010.19412207403770684No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5860.18927709714824661No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5790.18701610793316517No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5790.18701610793316517No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5750.18572411409597578No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5750.18572411409597578No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5730.18507811717738107No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5690.18378612334019165No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5520.17829514953213674No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5340.17248117726478446No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5310.1715121818868924No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5250.1695741911311083No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG5170.1669902034567295No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5070.16376021886375602No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5060.16343722040445866No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5030.1624682250265666No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4990.1611762311893772No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4900.15826924505570109No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4850.15665425275921432No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG4840.15633125429991698No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG4700.1518092758697541No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4640.14987128511397002No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4600.1485792912767806No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4530.14631830206169916No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG4500.14534930668380713No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4440.143411315928023No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4350.14050432979434688No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4330.13985833287575217No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG4330.13985833287575217No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG4330.13985833287575217No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG4330.13985833287575217No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4300.13888933749786014No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4190.13533635444558928No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG4180.13501335598629194No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4150.1340443606083999No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG4120.13307536523050784No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4100.13242936831191315No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG4080.13178337139331844No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG4030.1301683790968317No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4020.12984538063753437No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG4000.12919938371893966No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG4000.12919938371893966No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3980.12855338680034498No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3960.12790738988175027No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG3950.12758439142245293No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG3930.12693839450385822No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG3930.12693839450385822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3870.1250004037480741No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3860.12467740528877677No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG3770.12177041915510065No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3710.11983242839931653No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG3670.11854043456212714No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3630.11724844072493773No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3600.11627944534704569No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG3580.115633448428451No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG3580.115633448428451No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG3570.11531044996915364No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3530.11401845613196425No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3500.11304946075407221No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3480.1124034638354775No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3470.11208046537618016No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3460.1117574669168828No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3450.11143446845758545No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3420.11046547307969341No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3390.10949647770180136No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3370.10885048078320665No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3330.10755848694601726No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3310.10691249002742256No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3310.10691249002742256No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3300.10658949156812521No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3300.10658949156812521No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG3290.10626649310882788No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3290.10626649310882788No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG3220.10400550389374641No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA3160.10206751313796233No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG3150.10174451467866498No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3100.10012952238217825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)