FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257984
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG61132.369526792359216No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA52462.0334594393450756No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG49891.9338408583478044No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA42551.6493270900520964No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG30951.1996868022823122No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27351.060143264698586No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG24900.9651761349541057No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG23550.9128473083602083No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG21360.827958322996775No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG20340.7884209873480525No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG20160.7814438104688662No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG19030.7376426445050855No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG18140.7031443810468867No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA17420.6752356735301414No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA17250.6686461175886876No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA15910.616704911932523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA15590.6043010419250806No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG15580.603913420987348No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG14940.5791056809724634No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA14580.5651513272140908No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13260.5139853634333913No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA12670.49111572810716947No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA12390.48026234185065736No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG12260.47522326966013395No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11230.435298313073679No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11220.43491069213594646No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10860.4209563383775738No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10580.4101029521210618No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10070.3903342842967006No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9860.38219424460431656No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9250.3585493674026296No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9050.35079694864797817No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9040.3504093277102456No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8500.3294777970726867No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8300.3217253783180352No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA8260.3201748945671049No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG7880.30544529893326716No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG7860.30467005705780204No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG7830.3035071942446043No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7820.3031195733068717No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG7810.30273195236913913No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7770.3011814686182089No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA7690.2980805011163483No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7640.2961423964276855No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7590.29420429173902257No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7460.2891652195484991No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG7330.2841261473579757No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG7070.27404800297692883No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA7030.2724975192259985No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG6850.2655203423468122No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6850.2655203423468122No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6760.26203175390721906No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6550.25389171421483503No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6310.24458881170925328No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG6180.23954973951872985No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6030.23373542545274123No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG5850.22675824857355498No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5810.22520776482262464No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5700.22094393450756636No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG5610.21745534606797323No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG5560.21551724137931033No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5550.21512962044157777No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5500.21319151575291492No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5470.21202865293971718No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5320.20621433887372861No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5310.20582671793599605No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5270.2042762341850657No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5250.2035009923096006No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5210.2019505085586703No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5210.2019505085586703No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5080.19691143636814687No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5030.194973331679484No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4920.19070950136442572No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4870.18877139667576284No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4830.18722091292483256No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4820.18683329198709997No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4630.1794684941701811No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4600.17830563135698338No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4540.17597990573058794No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4430.17171607541552966No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4170.16163793103448276No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4150.1608626891590176No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4140.16047506822128504No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG4100.15892458447035476No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG4090.1585369635326222No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG4070.15776172165715702No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4070.15776172165715702No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG4010.15543599603076158No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3950.15311027040436617No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3940.15272264946663358No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3870.15000930290250558No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3850.14923406102704043No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3850.14923406102704043No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3830.1484588191515753No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3780.14652071446291243No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3670.14225688414785412No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3560.13799305383279584No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3520.13644257008186553No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3500.1356673282064004No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3480.13489208633093525No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG3400.13179111882907465No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG3380.13101587695360953No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3380.13101587695360953No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3320.1286901513272141No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3310.12830253038948153No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3300.12791490945174894No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3240.1255891838253535No Hit
GANTANTGGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG3210.1244263210121558No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG3160.12248821632349294No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3150.12210059538576036No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG3110.12055011163483006No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG3090.11977486975936492No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3080.11938724882163233No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG3050.11822438600843463No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2980.11551103944430663No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2930.11357293475564377No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2920.11318531381791119No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2900.11241007194244604No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2890.11202245100471347No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG2890.11202245100471347No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2850.11047196725378318No Hit
GANTANCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2850.11047196725378318No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2830.10969672537831804No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2830.10969672537831804No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG2820.10930910444058546No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTCCCTGAG2810.1089214835028529No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG2800.1085338625651203No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2780.10775862068965517No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2760.10698337881419004No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2740.10620813693872487No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA2740.10620813693872487No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2730.1058205160009923No Hit
GANTANTGGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG2730.1058205160009923No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG2720.10543289506325974No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA2690.10427003225006201No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2680.10388241131232945No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2680.10388241131232945No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2670.10349479037459687No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2640.10233192756139917No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2630.10194430662366658No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG2630.10194430662366658No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2630.10194430662366658No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG2610.10116906474820143No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG2580.10000620193500372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)