FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002463524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309555
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG106013.4245933678990808No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG85932.775920272649448No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG36121.1668362649609925No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG35491.1464844696419052No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA33761.090597793606952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG33651.0870443055353654No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30790.9946536156741128No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG29880.9652565779909871No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG27180.8780345980520423No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA26910.8693124000581479No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25630.8279627206796853No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23630.7633538466508375No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG22000.7106976143173265No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG18500.5976320847668427No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG18370.5934325079549676No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA18250.5895559755132367No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG18010.581802910629775No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG17570.5675889583434285No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG14990.4842435108462147No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG14830.47907480092390686No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA14810.47842871218361843No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG12840.4147889712652033No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG12450.4021902408295779No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11590.37440842499717336No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA11270.3640710051525577No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11130.35954838397053834No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11050.35696402900938445No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11030.35631794026909597No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10960.35405662967808627No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10560.3411348548723167No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10470.3382274555410186No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10350.3343509230992877No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10310.33305874561871074No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10080.3256287251053932No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9970.3220752370338066No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9890.3194908820726527No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9620.3107686840787582No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9500.3068921516370273No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8970.28977080001938266No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8690.28072555765534396No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8650.279433380174767No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8580.27717206958375734No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8480.2739416258823149No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8470.2736185815121707No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8390.2710342265510168No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7910.2555280967840933No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG7560.24422154382904493No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7380.23840674516644864No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7160.23129976902327537No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7130.23033063591284264No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6920.2235467041398136No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6720.2170858167369288No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC6670.21547059488620765No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6590.2128862399250537No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6540.21127101807433252No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6420.20739448563260166No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA6350.20513317504159195No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG6260.2022257757102938No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6080.1964109770476975No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6060.195764888307409No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG5930.1915653114955339No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5910.19091922275524545No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG5900.1905961783851012No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA5800.1873657346836588No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5790.18704269031351456No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG5670.1831661578717837No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG5640.18219702476135097No Hit
GACTACTGGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG5520.1783204923196201No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5510.17799744794947586No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5490.17735135920918738No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG5460.17638222609875467No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5440.1757361373584662No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG5360.17315178239731227No Hit
GANTANTGGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG5300.17121351617644684No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG5300.17121351617644684No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG5240.1692752499555814No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA5230.16895220558543717No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC5200.16798307247500444No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5190.1676600281048602No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5110.16507567314370628No Hit
GANTANCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5090.16442958440341782No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5080.16410654003327357No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5050.16313740692284084No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4990.1611991407019754No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG4920.15893783011096574No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG4920.15893783011096574No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4880.15764565263038877No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4870.15732260826024455No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4870.15732260826024455No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG4830.1560304307796676No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4800.15506129766923488No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG4720.15247694270808096No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG4670.15086172085735974No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4620.14924649900663856No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA4610.1489234546364943No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4500.1453699665649077No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4470.14440083345447496No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4460.14407778908433075No Hit
GANTANTGGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG4460.14407778908433075No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4450.1437547447141865No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG4370.14117038975303256No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4350.1405243010127441No Hit
GANTANAAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG4310.13923212353216713No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG4290.13858603479187867No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4250.1372938573113017No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG4180.13503254672029202No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG4170.13470950235014778No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4170.13470950235014778No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG4160.13438645798000357No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4150.13406341360985932No Hit
GACTACAAGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG4150.13406341360985932No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG4070.1314790586487054No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4070.1314790586487054No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG4070.1314790586487054No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4050.13083296990841692No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4010.12954079242783997No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3990.1288947036875515No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3930.12695643746668603No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3830.12372599376524365No Hit
GANTANCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3770.12178772754437822No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3770.12178772754437822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3770.12178772754437822No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG3770.12178772754437822No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3770.12178772754437822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3760.12146468317423398No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3750.12114163880408975No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG3740.1208185944339455No Hit
GANTANAAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG3720.12017250569365703No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3650.11791119510264736No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3640.11758815073250312No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3630.11726510636235886No Hit
GANTANAAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3630.11726510636235886No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3560.11500379577134918No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG3560.11500379577134918No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3470.11209639644005104No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3460.1117733520699068No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3390.10951204147889712No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG3330.10757377525803169No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3320.10725073088788745No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3280.1059585534073105No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3240.10466637592673354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3200.10337419844615658No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG3170.10240506533572387No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3150.1017589765954354No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA3140.10143593222529115No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA3140.10143593222529115No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3130.1011128878551469No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3120.10078984348500265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)