FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104837
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG25612.44283983708042No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24152.303576027547526No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG23962.2854526550740677No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22422.138557951868138No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA18631.7770443641080915No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA17741.6921506719955741No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15931.5195017026431508No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15231.4527313830040922No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG14231.3573452120911509No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG12471.1894655512843748No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9530.9090302088003281No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8990.8575216765073399No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG8840.8432137508703987No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8750.8346289954882341No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8430.804105420796093No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8310.79265908028654No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG7980.7611816438852695No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7920.7554584736304931No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG7840.7478275799574577No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG7530.718257866974446No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6560.6257332811888932No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6450.6152408023884697No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6350.6057021852971756No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6170.5885326745328462No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6050.5770863340232933No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5790.5522859295859286No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5690.5427473124946345No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5570.5313009719850815No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5530.527485525148564No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5500.5246239400211757No Hit
GANTANTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG5360.5112698760933639No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG5100.4864694716559993No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4960.47311540772818755No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG4880.4654845140551523No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4650.44354569474517586No Hit
GANTANAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG4440.4235145988534582No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4290.40920667321651705No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4250.40539122637999947No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4120.39299102416131715No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3950.37677537510611714No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3830.3653290345965642No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3790.36151358776004655No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG3650.3481595238322348No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG3350.3195436725583525No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3290.313820502303576No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3230.3080973320487996No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3230.3080973320487996No Hit
GACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG3220.30714347033967015No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3110.2966509915392466No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3050.2909278212844702No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2970.28329692761143493No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2930.2794814807749173No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2870.27375831052014077No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2840.27089672539275256No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG2820.2689890019744937No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG2810.2680351402653643No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2790.2661274168471055No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2760.26326583171971724No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2690.2565887997558114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2680.25563493804668197No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2660.2537272146284232No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2640.25181949121016434No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2480.23655770386409378No Hit
GANTANTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG2450.23369611873670557No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG2410.22988067190018788No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2370.22606522506367024No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2320.22129591651802324No Hit
GANTANAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG2280.21748046968150556No Hit
GACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG2240.21366502284498792No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2230.21271116113585853No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2200.2098495760084703No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2120.202218682335435No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2050.19554165037152915No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1980.18886461840762328No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1940.18504917157110562No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1910.18218758644371738No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1910.18218758644371738No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1900.18123372473458796No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1880.17932600131632914No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1800.17169510764329388No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1750.16692579909764682No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1730.165018075679388No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1730.165018075679388No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1710.1631103522611292No Hit
GANTANTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG1710.1631103522611292No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1710.1631103522611292No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1610.15357173516983508No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1610.15357173516983508No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1580.15071015004244684No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1560.14880242662418802No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1540.1468947032059292No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCANGNTTTCGAGCCTCAG1520.1449869797876704No Hit
GACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG1470.14021767124202333No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1470.14021767124202333No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1440.1373560861146351No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1440.1373560861146351No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGCGCCTCAG1430.1364022244055057No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1420.13544836269637628No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1410.13449450098724686No Hit
GANTANTGGGGTATCTAATNCTGTTCGCTCNCNANGNTTTCGAGCCTCNG1370.13067905415072922No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1360.1297251924415998No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGCGCCTCAG1350.1287713307324704No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.1287713307324704No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1340.12781746902334098No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1330.1268636073142116No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1320.12590974560508217No Hit
GANTANTGGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGCGCCTCAG1310.12495588389595277No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCAG1300.12400202218682335No Hit
GANTANTGGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGCGCCTCNG1290.12304816047769396No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1290.12304816047769396No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1290.12304816047769396No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1290.12304816047769396No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCANGNTTTCGAGCCTCAG1250.1192327136411763No Hit
GANTANTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1240.11827885193204689No Hit
GANTANTGGGGTATCTAATCNTGTTCGCTCNNNANGNTTTCGAGCCTCNG1230.11732499022291748No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGCGCCTCAG1220.11637112851378807No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.11637112851378807No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTCNNNANGNTTTCGAGCCTCAG1220.11637112851378807No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGCGCCTCAG1210.11541726680465865No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGNTTTCGAGCCTCAG1210.11541726680465865No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1200.11446340509552926No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1200.11446340509552926No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1200.11446340509552926No Hit
GANTANAAGGGTATNTAATCNTGTTCGCTCCCNACGCTTTCGAGCCTCAG1190.11350954338639983No Hit
GANTANAAGGGTATCTAATNNTGTTCGCTCNNNANGNTTTCGAGCCTCAG1180.11255568167727044No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGCGCCTCAG1180.11255568167727044No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCAG1180.11255568167727044No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCNG1170.11160181996814102No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTCNNNANGNTTTCGAGCCTCAG1160.11064795825901162No Hit
GANTANAAGGGTATNTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1150.1096940965498822No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCNG1150.1096940965498822No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCANGCTTTCGAGCCTCAG1140.10874023484075278No Hit
GANTANTGGGGTATNTAATCNTGTTCGCTCCCNACGCTTTCGAGCCTCAG1130.10778637313162338No Hit
GANTANTGGGGTATNTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.10683251142249396No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.10683251142249396No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.10683251142249396No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.10587864971336455No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.10492478800423514No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1100.10492478800423514No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCANGCTTTCGAGCCTCAG1100.10492478800423514No Hit
GANTANTGGGGTATCTAATCNTGTTCGCTCNNNANGNTTTCGAGCCTCAG1090.10397092629510574No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGCGCCTCNG1090.10397092629510574No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGCGCCTCAG1090.10397092629510574No Hit
GANTANAAGGGTATCTAATNCTGTTCGCTCNCNANGNTTTCGAGCCTCNG1080.10301706458597631No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGCGCCTCAG1080.10301706458597631No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGCGCCTCAG1080.10301706458597631No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGCGCCTCAG1070.10206320287684692No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1070.10206320287684692No Hit
GANTANAAGGGTATCTAATCNTGTTCGCTCNNNANGNTTTCGAGCCTCAG1060.1011093411677175No Hit
GACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG1060.1011093411677175No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1060.1011093411677175No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGCGCCTCAG1050.1001554794585881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)