FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120316
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG22441.8650886000199474No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG19891.6531467136540443No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG18351.5251504371820872No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC17261.4345556700688187No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG16151.3422986136507198No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC14891.2375743874463914No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11910.98989328102663No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11150.9267262874430665No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG10950.9101033943947604No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG10370.8618970045546727No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9750.8103660361049237No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG9670.8037168788856013No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9520.7912497090993718No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG9130.7588350676551747No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8150.6773828917184747No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG8130.6757206024136441No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8040.6682403005419063No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8030.667409155889491No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7780.6466305395791083No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7470.6208650553542339No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7380.6133847534824961No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7170.5959307157817746No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG6630.5510489045513481No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6350.5277768542837196No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6120.5086605272781675No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG6100.5069982379733369No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5820.4837261877057083No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5620.4671032946574022No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5560.46211642674291037No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5430.4513115462615114No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5160.4288706406462981No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5110.4247149173842215No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG5010.41640347086006846No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4330.35988563449582767No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4240.3524053326240899No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4120.3424315967951062No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG4100.3407693074902756No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4010.33328900561853786No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3960.32913328235646133No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3960.32913328235646133No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3880.32248412513713887No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3850.3199906911798929No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3830.31832840187506234No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3740.3108481000033246No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3650.3033677981315868No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3430.28508261577845007No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3380.28092689251637354No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3290.2734465906446358No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3280.2726154459922205No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3270.2717843013398052No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3260.2709531566873899No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3250.27012201203497455No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3250.27012201203497455No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3250.27012201203497455No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3240.26929086738255925No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3190.2651351441204827No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3050.25349911898666844No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3030.25183682968183785No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3020.25100568502942255No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3010.2501745403770072No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2960.24601881711493068No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2890.24020080454802353No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2850.2368762259383623No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2830.2352139366335317No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2820.2343827919811164No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2770.23022706871903986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2730.22690249010937866No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2690.2235779114997174No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2640.21942218823764087No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2560.21277303101831843No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2560.21277303101831843No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2430.20196815053691947No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2400.1994747165796735No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2380.19781242727484294No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2370.19698128262242762No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2370.19698128262242762No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2340.19448784866518168No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2260.18783869144585924No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2210.18368296818378269No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2170.18035838957412148No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2140.17786495561687557No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2130.17703381096446028No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2060.1712157983975531No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2000.16622893048306128No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1980.16456664117823067No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1960.16290435187340005No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.16290435187340005No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1950.16207320722098476No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1890.1570863393064929No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1880.1562551946540776No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1870.1554240500016623No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1840.15293061604441635No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1810.15043718208717047No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1810.15043718208717047No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1790.14877489278233982No Hit
GANTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1760.14628145882509394No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1760.14628145882509394No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1750.14545031417267862No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1730.143788024867848No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1730.143788024867848No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1710.14212573556301739No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1630.13547657834369495No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1630.13547657834369495No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1620.13464543369127963No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1590.13215199973403371No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1580.1313208550816184No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1570.1304897104292031No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1560.1296585657767878No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1550.12882742112437248No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1540.1279962764719572No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1510.12550284251471128No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1500.12467169786229595No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1460.12134711925263474No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1430.11885368529538881No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1430.11885368529538881No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1420.11802254064297349No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1420.11802254064297349No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1400.11636025133814289No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCANGNTTTCGAGCCTCAG1380.11469796203331228No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1360.11303567272848167No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1360.11303567272848167No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1360.11303567272848167No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1360.11303567272848167No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCANGCTTTCGAGCCTCAG1350.11220452807606636No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1350.11220452807606636No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1350.11220452807606636No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1350.11220452807606636No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.11054223877123574No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1320.10971109411882043No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1310.10887994946640514No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1300.10804880481398982No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1300.10804880481398982No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1300.10804880481398982No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.10804880481398982No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1290.10721766016157452No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1280.10638651550915922No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.10638651550915922No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.10638651550915922No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1240.103061936899498No Hit
GANTANAAGGGTATCTAATNNTGTTCGCTANNNANGNTTTCGAGCCTCAG1240.103061936899498No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1240.103061936899498No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1230.10223079224708267No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1220.10139964759466738No Hit
GANTANTGGGGTATNTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1210.10056850294225207No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGAGCCTCAG1210.10056850294225207No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1210.10056850294225207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)