FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101229
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG17861.7643165496053503No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG17311.709984293038556No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA16311.6111983720080214No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA15671.5479753825484792No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14121.3948572049511503No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13171.3010105799721423No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA12741.2585326339290124No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA11971.1824674747355006No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG9000.8890732892748126No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG7470.7379308300980945No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG7140.705331476158018No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG6740.665817107745804No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6720.6638413893251933No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6320.6243270209129794No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6130.6055576959171779No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6030.5956791038141244No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6010.5937033853935137No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5980.5907398077625977No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5820.5749340603977121No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5620.5551768761916052No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5580.5512254393503837No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5430.5364075511958036No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5340.5275168183030554No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5240.517638226200002No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5210.514674648569086No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5190.5126989301484752No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5120.5057839156763378No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4860.48009957620839877No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4810.47516028015687206No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4700.4642938288435133No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4670.46133025121259713No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4620.4563909551610705No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4430.43762163016526884No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4420.4366337709549635No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4150.40996157227671914No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4010.3961315433324442No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3920.38724081043969616No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3560.3516778788687036No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3480.34377500518626086No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3370.332908553872902No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3350.33093283545229135No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3170.3131513696667951No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3070.3032727775637416No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2990.29536990388129886No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2920.2884548894091614No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2900.2864791709885507No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2820.27857629730610795No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2790.27561271967519185No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2750.2716612828339705No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2740.27067342362366514No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2710.2677098459927491No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2650.26178269073091703No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2630.2598069723103063No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2560.2528919578381689No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2560.2528919578381689No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2470.24400122494542079No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2450.24202550652481009No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2440.24103764731450475No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2390.23609835126297798No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2290.22621975915992454No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2280.22523189994961917No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2270.22424404073931384No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2250.22226832231870314No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG2230.22029260389809244No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2220.2193047446877871No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2130.210414011795039No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2110.2084382933744283No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG2080.20547471574351223No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2070.2044868565332069No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG2040.20152327890229083No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2040.20152327890229083No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG2030.2005354196919855No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2000.19757184206106948No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2000.19757184206106948No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1970.19460826443015342No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1940.19164468679923738No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1940.19164468679923738No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1870.18472967232709994No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG1860.1837418131167946No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1860.1837418131167946No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1850.18275395390648924No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1840.1817660946961839No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1800.1778146578549625No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1790.17682679864465717No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG1760.17386322101374113No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1740.17188750259313043No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1710.1689239249622144No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1690.1669482065416037No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG1690.1669482065416037No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1690.1669482065416037No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1660.16398462891068763No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1660.16398462891068763No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1650.16299676970038232No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1630.16102105127977162No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1630.16102105127977162No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1550.15311817759732885No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCAG1540.15213031838702348No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1520.15015459996641278No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1470.14521530391488605No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1440.14225172628397No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1420.1402760078633593No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1400.1383002894427486No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1380.13632457102213794No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.13632457102213794No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1380.13632457102213794No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1360.13434885260152724No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1360.13434885260152724No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1350.13336099339122187No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1350.13336099339122187No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1340.13237313418091654No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1330.1313852749706112No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1320.13039741576030583No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1320.13039741576030583No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1310.1294095565500005No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1280.12644597891908446No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1270.1254581197087791No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1260.12447026049847375No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1230.12150668286755771No Hit
GANTANTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1230.12150668286755771No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.12051882365725237No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1200.11854310523664167No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1190.11755524602633632No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1180.11656738681603097No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1180.11656738681603097No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1160.11459166839542029No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1150.11360380918511494No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1140.11261594997480959No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1130.11162809076450424No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1130.11162809076450424No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1110.10965237234389355No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1100.10866451313358821No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1100.10866451313358821No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1090.10767665392328286No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1090.10767665392328286No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.10668879471297751No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1070.10570093550267216No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1050.10372521708206146No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1040.10273735787175611No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1040.10273735787175611No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1040.10273735787175611No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.10273735787175611No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.10174949866145076No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1030.10174949866145076No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1020.10076163945114541No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1020.10076163945114541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)