FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81144
Sequences flagged as poor quality0
Sequence length165-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT49616.113822340530415No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC35344.355220349009168No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC33114.080400276052449No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23392.8825298235236123No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23322.8739031844621907No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT21802.6865818791284632No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC19662.4228531992507145No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC16672.054372473627132No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC15931.9631765749778172No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT15061.8559597752144337No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT12921.5922310953366852No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT12471.5367741299418318No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11501.4172335600907031No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11321.3950507739327616No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11031.3593118406783002No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9941.2249827467218772No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC9691.194173321502514No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC9591.1818495514147689No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC9451.1645962732919255No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8651.0660061125899634No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC8271.0191757862565316No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT6840.8429458740017746No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6700.8256925958789314No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6290.7751651385191758No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6210.7653061224489796No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6190.7628413684314306No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6010.7406585822734891No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5910.7283348121857438No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGGAACTCTGAC5660.6975253869663808No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC5350.6593216996943705No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5220.6433007985803016No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5190.6396036675539781No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4840.5964704722468697No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT4630.5705905550626047No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4370.5385487528344671No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4250.5237602287291728No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT4020.4954155575273588No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA3900.4806270334220645No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA3790.4670708863255447No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC3770.4646061323079957No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC3640.4485852311939268No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC3620.44612047717637776No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC3560.43872621512373067No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3040.3746426106674554No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3020.3721778566499064No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2930.36108646357093566No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGGAACTCTGAC2870.35369220151828845No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC2770.3413684314305432No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2770.3413684314305432No Hit
CCTANGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT2660.32781228433402343No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2660.32781228433402343No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC2640.3253475303164744No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2610.3216503992901508No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC2600.32041802228137634No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2560.3154885142462782No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2560.3154885142462782No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA2500.30809425219363107No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA2410.29700285911466034No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2390.2945381050971113No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2350.2896085970620132No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2290.28221433500936605No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2220.27358769594794435No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2190.26989056492162083No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC2170.26742581090407175No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA2120.26126392586019914No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2120.26126392586019914No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1960.24154589371980675No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1890.2329192546583851No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1890.2329192546583851No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1860.22922212363206151No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGAGGCAACTCTGAC1840.22675736961451248No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1830.22552499260573797No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1750.21566597653554176No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1740.21443359952676724No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1590.19594794439514937No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1540.18978605935127674No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1490.18362417430740413No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1480.1823917972986296No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1480.1823917972986296No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1470.18115942028985507No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1450.178694666272306No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGGAACTCTGAC1420.17499753524598247No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1400.1725327812284334No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1350.16637089618456077No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGGAACTCTGAC1340.16513851917578626No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1310.16144138814946268No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1300.16020901114068817No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC1260.15527950310559005No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1230.15158237207926648No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1220.15034999507049196No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1200.14788524105294293No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1200.14788524105294293No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA1170.14418811002661935No Hit
CCTACGGGTGGCAGCAGTCGAGAATCCTTCACAATGGGGGCAACCCTGAT1170.14418811002661935No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1170.14418811002661935No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1170.14418811002661935No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1130.13925860199152124No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1130.13925860199152124No Hit
CCTANGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT1100.13556147096519766No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1100.13556147096519766No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1090.13432909395642315No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1090.13432909395642315No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1080.13309671694764863No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA1070.13186433993887411No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1060.13063196293009957No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1060.13063196293009957No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1060.13063196293009957No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1040.12816720891255054No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA1030.126934831903776No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGAGAGACTGAA1030.126934831903776No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGGCAATGGAGGCAACTCTGAC1030.126934831903776No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC990.1220053238686779No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA970.11954056985112886No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA970.11954056985112886No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC960.11830819284235432No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT950.1170758158335798No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC940.11584343882480529No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT930.11461106181603076No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA930.11461106181603076No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC890.10968155378093267No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC880.10844917677215814No Hit
CCTANGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT880.10844917677215814No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC880.10844917677215814No Hit
CCTACGGGTGGCAGCAGTCGCGAATCCTTCACAATGGGGGCAACCCTGAT820.101054914719511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)