FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81144
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG24723.046435965690624No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG19162.3612343488119887No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13241.63166715961747No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11281.3901212658976634No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG8491.0462880804495711No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8231.0142462782214334No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7970.9822044759932959No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG7720.9513950507739328No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG7600.9366065266686384No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7290.8984028393966283No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6940.8552696440895199No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6680.8232278418613822No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6120.7542147293700089No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4930.6075618653258404No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4920.6063294883170659No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4580.5644286700187321No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4420.5447106378783397No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG4400.5422458838607906No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4330.533619244799369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4270.5262249827467219No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG4150.5114364586414276No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3950.48678891846593714No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3740.46090900128167206No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG3680.45351473922902497No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3670.4522823622202504No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3640.4485852311939268No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG3590.44242334615005424No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3470.42763482204475994No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3420.4214729370008873No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3390.41777580597456376No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3310.4079167899043675No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3160.38943113477274965No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3120.3845016267376516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3070.378339741693779No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3070.378339741693779No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3040.3746426106674554No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3030.37341023365868087No Hit
GANTANTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2850.3512274475007394No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2850.3512274475007394No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2660.32781228433402343No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2620.32288277629892537No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2520.3105590062111801No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2510.3093266292024056No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2440.3006999901409839No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2420.29823523612343483No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2380.29330572808833677No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2360.29084097407078774No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2330.28714384304446416No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2310.2846790890269151No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2250.277284826974268No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2180.2686581879128463No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2140.26372867987774823No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1960.24154589371980675No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1950.24031351671103224No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1940.23908113970225772No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1920.23661638568470864No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1880.23168687764961057No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1870.23045450064083603No Hit
GANTANACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1740.21443359952676724No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1720.21196884550921816No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1700.20950409149166913No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1670.20580696046534555No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1650.20334220644779652No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1630.2008774524302475No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1620.19964507542147292No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1600.19718032140392389No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1570.1934831903776003No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1510.1860889283249532No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG1510.1860889283249532No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1500.18485655131617865No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1490.18362417430740413No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1470.18115942028985507No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1460.17992704328108053No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1440.1774622892635315No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1440.1774622892635315No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1420.17499753524598247No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1390.1713004042196589No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.17006802721088435No Hit
GANTANTGGGGTATCTAATNNCTTTCGCTCNNNTNGNCTTCGTGCCTCAG1380.17006802721088435No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1370.16883565020210983No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1360.1676032731933353No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1330.16390614216701171No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1330.16390614216701171No Hit
GANTANAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1310.16144138814946268No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTNGCCTTCGTGCCTCAG1300.16020901114068817No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1300.16020901114068817No Hit
GANTANTGGGGTATNTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1280.1577442571231391No Hit
GANTANTGGGGTATNTAATNNCTTTCGNTCNNNTNGNCTTCGTGCCTCAG1270.1565118801143646No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1260.15527950310559005No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG1260.15527950310559005No Hit
GANTANCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1250.15404712609681553No Hit
GANTANTGGGGTATNTAATCNCTTTCGCTCCCNTGGCCTTCGTGCCTCAG1240.15281474908804102No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1240.15281474908804102No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1240.15281474908804102No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1230.15158237207926648No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.15034999507049196No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1220.15034999507049196No Hit
GANTANTGGGGTATCTAATCNCTTTCGCTCNNNTNGNCTTCGTGCCTCNG1220.15034999507049196No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1210.14911761806171742No Hit
GANTANAAGGGTATCTAATCCCTTTCGNTNNCCTNGNCTTCGTGCCTCAG1170.14418811002661935No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG1160.1429557330178448No Hit
GANTANTGGGGTATCTAATCCCTTTCGNTNNCCTNGNCTTCGTGCCTCAG1150.1417233560090703No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1140.14049097900029578No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG1140.14049097900029578No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGNCTTCGTGCCTCAG1140.14049097900029578No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1140.14049097900029578No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.13925860199152124No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1120.13802622498274672No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.13802622498274672No Hit
GANTANTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1110.13679384797397218No Hit
GANTANTGGGGTATCTAATCNCTTTCGCTCNNNTNGNCTTCGTGCCTCAG1110.13679384797397218No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1100.13556147096519766No Hit
GANTANAAGGGTATNTAATNNCTTTCGNTCNNNTNGNCTTCGTGCCTCAG1100.13556147096519766No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1090.13432909395642315No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1080.13309671694764863No Hit
GANTANAAGGGTATCTAATNCCTTTCGCTCNCNTNGNCTTCGTGCCTCNG1080.13309671694764863No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1070.13186433993887411No Hit
GANTANAAGGGTATCTAATNNCTTTCGCTCNNNTNGNCTTCGTGCCTCAG1050.12939958592132506No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1050.12939958592132506No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.126934831903776No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG1030.126934831903776No Hit
GANTANAAGGGTATNTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1010.12447007788622694No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1000.12323770087745242No Hit
GANTANAAGGGTATCTAATCNCTTTCGCTCNNNTNGNCTTCGTGCCTCNG1000.12323770087745242No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1000.12323770087745242No Hit
GANTANAAGGGTATNTAATCNCTTTCGCTCCCNTGGCCTTCGTGCCTCAG990.1220053238686779No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC990.1220053238686779No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC980.12077294685990338No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.12077294685990338No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG970.11954056985112886No Hit
GANTANTGGGGTATCTAATNCCTTTCGCTCNCNTNGNCTTCGTGCCTCNG970.11954056985112886No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA960.11830819284235432No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG950.1170758158335798No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG950.1170758158335798No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG950.1170758158335798No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG940.11584343882480529No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG940.11584343882480529No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG930.11461106181603076No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG930.11461106181603076No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG900.11091393078970718No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG900.11091393078970718No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTNGCCTTCGTGCCTCAG880.10844917677215814No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA880.10844917677215814No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG870.10721679976338362No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG860.10598442275460908No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG850.10475204574583456No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG840.10351966873706005No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG830.10228729172828552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG830.10228729172828552No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCCTTCGTGCTTGAG820.101054914719511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)