FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86399
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG15951.8460861815530274No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG14011.621546545677612No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13511.563675505503536No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12321.425942429889235No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12091.3993217514091598No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9611.1122813921457424No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9321.0787161888447783No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7770.8993159643051424No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG6010.6956099028923945No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG5640.6527853331635783No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5590.6469982291461707No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5460.6319517587009109No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5380.6226923922730587No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5270.6099607634347619No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5090.5891271889720946No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5070.5868123473651315No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4990.5775529809372794No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG4770.5520897232606858No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG4600.5324135696015No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4420.5115799951388327No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4420.5115799951388327No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4310.4988483663005359No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4210.48727415826572074No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4080.4722276878204609No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4010.4641257421960902No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3800.43981990532297827No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3770.43634764291253375No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3740.4328753805020891No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG3620.41898633086031084No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG3510.40625470202201414No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3460.4004675980046065No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3390.39236565238023585No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3320.3842637067558652No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3240.37500434032801305No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3190.36921723631060543No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3140.3634301322931978No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3060.3541707658653457No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3050.35301334506186416No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2990.346068820240975No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2950.3414391370270489No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2930.3391242954200859No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2850.3298649289922337No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2830.32755008738527064No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2810.32523524577830765No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2780.3217629833678631No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2610.3020868297086772No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2600.3009294089051957No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG2460.2847255176564544No Hit
GANTANTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2310.2673642056042315No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2200.25463257676593476No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.25231773515897177No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2180.25231773515897177No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2150.2488454727485272No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2080.2407435271241565No Hit
GANTANAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2050.237271264713712No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG1950.22569705667889675No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1910.22106737346497068No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1900.21990995266148913No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG1890.2187525318580076No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1890.2187525318580076No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1870.21643769025104456No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.21528026944756304No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1820.21065058623363697No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1820.21065058623363697No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1810.20949316543015542No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1790.2071783238231924No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.2013912198057848No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1670.19328927418141414No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1670.19328927418141414No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1660.1921318533779326No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1660.1921318533779326No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1650.1909744325744511No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1640.18981701177096957No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1640.18981701177096957No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1630.18865959096748805No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1630.18865959096748805No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG1610.186344749360525No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1590.18402990775356196No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG1540.17824280373615436No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.17592796212919132No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1520.17592796212919132No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1510.1747705413257098No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG1490.17245569971874675No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1480.17129827891526522No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1470.1701408581117837No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1460.16898343730830218No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1460.16898343730830218No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1440.16666859570133913No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1390.16088149168393154No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1380.15972407088045001No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG1380.15972407088045001No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1350.15625180847000544No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.15277954605956087No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1290.1493072836491163No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1280.14814986284563478No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG1280.14814986284563478No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1270.14699244204215328No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1270.14699244204215328No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1260.14583502123867173No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1260.14583502123867173No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1240.1435201796317087No Hit
GACTACAGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG1230.1423627588282272No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1220.14120533802474564No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1210.14004791722126414No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1200.13889049641778262No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1180.13657565481081957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1180.13657565481081957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1160.13426081320385652No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1150.133103392400375No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1150.133103392400375No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1150.133103392400375No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1110.1284737091864489No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1100.12731628838296738No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.12500144677600436No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1070.12384402597252282No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1070.12384402597252282No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1060.12268660516904131No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.11805692195511522No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1010.11689950115163371No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1010.11689950115163371No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1000.11574208034815217No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG990.11458465954467065No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.11342723874118914No Hit
GACTACCAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG970.11226981793770763No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG960.11111239713422609No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG940.10879755552726306No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA930.10764013472378152No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG930.10764013472378152No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.1064827139203No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA920.1064827139203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG920.1064827139203No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCATCAG920.1064827139203No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA920.1064827139203No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG910.10532529311681849No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG910.10532529311681849No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG900.10416787231333696No Hit
GACTACTCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG900.10416787231333696No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.10416787231333696No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG890.10301045150985542No Hit
GANTANACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG890.10301045150985542No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG880.10185303070637391No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA880.10185303070637391No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.10185303070637391No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC870.1006956099028924No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG870.1006956099028924No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG870.1006956099028924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)