FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79616
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA48836.133189308681672No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC38714.862088022508038No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT32494.080838022508038No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA28413.5683782154340835No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA24113.02828577170418No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC21382.6853898713826365No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC17182.157857717041801No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA15411.9355405948553055No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA14201.7835610932475883No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14131.774768890675241No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12181.5298432475884245No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC12021.5097467845659165No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC10441.3112942122186495No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT10321.2962218649517685No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10121.2711012861736335No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA10011.2572849678456592No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC8831.1090735530546623No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC8411.0563203376205788No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8001.004823151125402No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC7850.9859827170418006No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT7820.9822146302250805No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA7560.9495578778135049No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA7440.9344855305466239No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA7240.9093649517684886No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT6760.8490755627009646No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6380.801346463022508No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6110.7674336816720256No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT5830.7322648713826366No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5830.7322648713826366No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC5760.7234726688102894No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5670.7121684083601286No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5290.6644393086816721No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA5080.6380627009646302No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT5060.6355506430868167No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4350.5463725884244373No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA4340.5451165594855306No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4290.5388364147909968No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC4200.527532154340836No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4190.5262761254019293No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC4100.5149718649517685No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC4060.5099477491961415No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA3750.47101085209003213No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA3680.4622186495176849No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3600.4521704180064309No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA3390.42579381028938906No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3350.420769694533762No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT3210.4031852893890675No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3210.4031852893890675No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3180.39941720257234725No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3040.38183279742765275No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA3000.37680868167202575No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2970.3730405948553055No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2740.34415192926045013No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2710.3403838424437299No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT2640.3315916398713826No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA2640.3315916398713826No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2550.3202873794212219No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2510.3152632636655949No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2430.3052150321543408No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2370.2976788585209003No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2300.28888665594855306No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2280.28637459807073956No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2260.283862540192926No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC2250.2826065112540193No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2240.28135048231511256No Hit
CCTANGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2230.2800944533762058No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2120.2662781350482315No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2000.2512057877813505No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1970.24743770096463022No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1970.24743770096463022No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1940.24366961414790997No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1920.2411575562700965No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1860.23362138263665597No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC1840.23110932475884244No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1830.2298532958199357No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1820.22859726688102897No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC1780.22357315112540194No Hit
CCTANGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1770.22231712218649516No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGGCGAAAGCCTGAC1730.21729300643086816No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1710.21478094855305463No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1700.2135249196141479No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1680.21101286173633443No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1530.19217242765273312No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1520.19091639871382637No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1470.1846362540192926No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT1370.17207596463022506No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1360.17081993569131834No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1350.16956390675241156No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1340.16830787781350484No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1340.16830787781350484No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1310.16453979099678456No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1270.15951567524115756No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1260.1582596463022508No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1240.1557475884244373No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1230.15449155948553053No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1230.15449155948553053No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1210.15197950160771703No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1140.14318729903536978No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1090.136907154340836No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1070.1343950964630225No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1070.1343950964630225No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT1040.13062700964630225No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1030.1293709807073955No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1000.12560289389067525No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1000.12560289389067525No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT1000.12560289389067525No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1000.12560289389067525No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT970.12183480707395498No Hit
CCTACGGGTGGCAGCAGTCGAGAATCCTTCACAATGGGGGAAACCCTGAT970.12183480707395498No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT970.12183480707395498No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC930.11681069131832798No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC910.11429863344051448No Hit
CCTANGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC900.11304260450160772No Hit
CCTANGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC890.11178657556270097No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA880.11053054662379422No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA870.10927451768488745No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA870.10927451768488745No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC860.10801848874598072No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA810.10173834405144695No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT800.10048231511254019No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA800.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)