FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79616
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG22372.809736736334405No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG20262.5447146302250805No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG14721.8488745980707395No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG13851.739600080385852No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG12991.6315815916398715No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG11761.4770900321543408No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG10561.3263665594855305No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG9321.1706189710610932No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9171.151778536977492No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG9001.1304260450160772No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8871.1140976688102895No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG8851.111585610932476No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG7780.9771905144694534No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7530.9457897909967846No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7380.9269493569131833No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG7130.8955486334405145No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6700.841539389067524No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG5700.7159364951768489No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG4990.6267584405144694No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4940.6204782958199356No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4790.6016378617363344No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG4690.5890775723472669No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4680.5878215434083601No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4540.5702371382636656No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4110.5162278938906752No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4010.5036676045016077No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3760.4722668810289389No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3570.4484023311897106No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3490.43835409967845657No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG3400.4270498392282958No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3320.4170016077170418No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG3310.41574557877813506No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3240.4069533762057878No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3200.40192926045016075No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG3130.3931370578778135No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3020.3793207395498392No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2970.3730405948553055No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2930.36801647909967844No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG2900.3642483922829582No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2810.35294413183279744No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2770.34792001607717044No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2740.34415192926045013No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2580.3240554662379421No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2570.3227994372990354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2470.3102391479099678No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2330.2926547427652733No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2290.2876306270096463No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2280.28637459807073956No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2240.28135048231511256No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2160.27130225080385856No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2140.268790192926045No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2100.263766077170418No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2100.263766077170418No Hit
GANTANTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2090.26251004823151125No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2090.26251004823151125No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1960.2461816720257235No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1890.23738946945337622No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1880.23613344051446944No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1830.2298532958199357No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1810.22734123794212216No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1760.22106109324758844No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1750.21980506430868169No Hit
GANTANTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1750.21980506430868169No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1730.21729300643086816No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1720.21603697749196143No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1680.21101286173633443No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1660.2085008038585209No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1620.2034766881028939No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1560.19594051446945338No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1550.19468448553054662No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1480.18589228295819937No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1480.18589228295819937No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1470.1846362540192926No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1430.1796121382636656No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1420.17835610932475884No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1410.1771000803858521No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1400.17584405144694534No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1390.17458802250803857No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1380.17333199356913184No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1350.16956390675241156No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1340.16830787781350484No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1330.16705184887459806No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1300.1632837620578778No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1300.1632837620578778No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1290.16202773311897106No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1290.16202773311897106No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1290.16202773311897106No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1280.1607717041800643No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.15951567524115756No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1260.1582596463022508No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1260.1582596463022508No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1230.15449155948553053No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1210.15197950160771703No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1200.15072347266881028No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1190.14946744372990353No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1180.14821141479099678No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1180.14821141479099678No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1180.14821141479099678No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1170.14695538585209003No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.14569935691318328No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1160.14569935691318328No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1150.14444332797427653No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1150.14444332797427653No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1140.14318729903536978No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1120.14067524115755628No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1100.13816318327974278No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1100.13816318327974278No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1090.136907154340836No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1090.136907154340836No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1080.13565112540192928No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1050.131883038585209No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1050.131883038585209No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1050.131883038585209No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1050.131883038585209No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1020.12811495176848875No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1020.12811495176848875No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTNGCTTTCGCATCTCAG1010.12685892282958197No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.12560289389067525No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG990.12434686495176848No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG990.12434686495176848No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG990.12434686495176848No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG980.12309083601286175No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG960.12057877813504825No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG950.11932274919614148No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG940.11806672025723472No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG920.11555466237942122No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG900.11304260450160772No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG900.11304260450160772No Hit
GANTANAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG870.10927451768488745No Hit
GANTANTGGGGTATNTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG860.10801848874598072No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG850.10676245980707395No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG840.10550643086816722No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGNTTTCGCATCTCAG840.10550643086816722No Hit
GANTANAAGGGTATNTAATCNCGTTCGCTACCNTGGCTTTCGCATCTCAG830.10425040192926045No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGTGCATCAG800.10048231511254019No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG800.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)