FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114364
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT61785.402049596026721No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA56024.8983945996992055No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT35193.0770172431884157No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT28142.460564513308384No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA27852.43520688328495No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC26082.2804379000384736No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC25862.261201077262075No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA22771.9910111573572105No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT21111.8458605854989332No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18891.65174355566437No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA17201.5039697807002204No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16801.4689937392885872No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA16671.4576265258298067No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT16351.4296456927005001No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT15361.3430799902067085No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13921.2171662411248296No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA13861.2119198349130844No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC13231.1568325696897626No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC12211.0676436640900984No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC11801.0317932216431744No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA10560.923367493267112No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT9970.8717778321849534No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT9700.848169004232101No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC9570.8368017907733203No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9530.8333041866321569No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA9220.8061977545381414No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8730.763352103808891No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8520.7449896820677836No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8170.7143856458326047No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC7550.6601727816445735No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC7540.6592983806092826No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC7210.6304431464446855No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA6760.5910950998565982No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6530.5709838760449093No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA6460.5648630687978735No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6380.5578678605155468No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5830.5097758035745514No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT5750.5027805952922247No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5690.4975341890804798No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC5650.49403658493931657No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5610.4905389807981533No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA5530.48354377251582664No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC5520.4826693714805358No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5390.47130215802175507No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5300.4634325487041377No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5240.4581861424923927No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA5020.4389493197159945No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC4520.3952292679514533No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4480.3917316638102899No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA4410.38561085656325417No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT4310.3768668462103459No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4290.37511804413976424No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4280.37424364310447344No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4270.37336924206918265No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTGGGCAATGCGCGAAAGCGTGAC4190.366374033786856No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT4090.3576300234339478No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4080.35675562239865694No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3860.3375187996222587No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC3750.3279003882340597No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA3700.3235283830576055No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3620.3165331747752789No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3510.30691476338707985No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC3440.30079395614004406No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3400.29729635199888077No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC3400.29729635199888077No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3340.2920499457871358No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3280.28680353957539084No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3190.2789339302577734No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGAC3170.27718512818719176No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3140.2745619250813193No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2870.25095309712846703No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC2870.25095309712846703No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2760.2413346857402679No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2730.23871148263439543No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2730.23871148263439543No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2690.23521387849323216No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2680.23433947745794131No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2610.22821867021090553No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT2490.2177258577874156No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2490.2177258577874156No Hit
CCTACGGGTGGCAGCAGTTTCGAATCATTCACAATGGGGGCAACCCTGAT2450.2142282536462523No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC2430.21247945157567066No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC2420.2116050505403798No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA2320.2028610401874716No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA2310.20198663915218076No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2310.20198663915218076No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2280.19936343604630827No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2250.1967402329404358No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTGGGCAATGCGCGAAAGCGTGAC2210.1932426287992725No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2150.18799622258752755No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT2120.18537301948165508No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2120.18537301948165508No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC2110.18449861844636423No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2090.1827498163757826No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC2070.18100101430520094No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2050.1792522122346193No Hit
CCTACGGGAGGCTGCAGTCGAGAATTTTGGGCAATGGGGGCAACCCTGAC2030.17750341016403764No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1950.17050820188171104No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGGCAATGGGCGAAAGCCTGAC1900.1661361967052569No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT1900.1661361967052569No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC1880.16438739463467525No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1850.16176419152880275No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1840.16088979049351193No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1840.16088979049351193No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1790.15651778531705782No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1760.15389458221118532No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1760.15389458221118532No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1730.15127137910531285No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1670.1460249728935679No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1630.1425273687524046No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1620.14165296771711378No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1600.13990416564653213No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1580.13815536357595049No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1540.13465775943478717No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1500.13116015529362385No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1490.13028575425833305No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1490.13028575425833305No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1490.13028575425833305No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1480.12941135322304223No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1450.12678815011716973No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGAC1440.1259137490818789No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA1440.1259137490818789No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1390.1215417439054248No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1360.11891854079955232No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1360.11891854079955232No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1350.11804413976426148No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1220.10667692630548076No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1220.10667692630548076No Hit
CCTACGGGTGGCTGCAGTCGAGAATTTTGGGCAATGGGGGCAACCCTGAC1200.1049281242348991No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1190.10405372319960828No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1170.10230492112902662No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1160.1014305200937358No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC1150.10055611905844496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)