FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158480
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG37492.365598182735992No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG33632.12203432609793No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA28711.8115850580514892No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG27351.7257698132256436No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA27211.7169358909641594No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG25331.598308934881373No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13450.8486875315497223No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG13110.827233720343261No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG12680.8001009591115598No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG12570.7931600201918224No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG12540.7912670368500758No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG12190.7691822311963654No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11600.7319535588086825No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA11200.7067137809187279No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11010.6947248864209995No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10830.68336698637052No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10440.6587582029278142No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG10130.6391973750630995No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10120.6385663806158506No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10010.6316254416961131No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9710.6126956082786471No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9530.6013377082281676No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG9490.5988137304391721No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9440.5956587582029278No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG9340.5893488137304391No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7710.48649671882887424No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6830.4309692074709742No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6460.4076224129227663No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6280.39626451287228676No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5930.37417970721857646No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5660.35714285714285715No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5630.35524987380111056No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5610.3539878849066128No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG5570.3514639071176174No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGTCTCAG5350.33758202927814235No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5270.3325340737001514No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGTCTCAG5180.32685512367491165No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5130.3237001514386673No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5100.3218071680969208No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4990.3148662291771832No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4920.3104492680464412No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG4720.2978293791014639No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG4720.2978293791014639No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4690.2959363957597173No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4690.2959363957597173No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4620.2915194346289753No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4470.28205451792024233No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4420.278899545683998No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4320.2725896012115093No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4310.2719586067642605No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4300.27132761231701163No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4110.2593387178192832No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4050.25555275113579No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3940.2486118122160525No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3910.24671882887430588No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3890.24545683997980816No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3840.24230186774356388No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3830.24167087329631498No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3800.23977788995456842No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3790.23914689550731955No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3740.2359919232710752No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3710.23409893992932862No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3690.2328369510348309No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3670.23157496214033318No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3610.22778899545684No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG3520.2221100454316002No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3410.2151691065118627No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3350.2113831398283695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3220.20318021201413428No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3140.19813225643614338No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3080.1943462897526502No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2860.18046441191317517No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2850.1798334174659263No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2780.17541645633518424No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG2770.1747854618879354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2770.1747854618879354No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2730.17226148409893993No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2630.1659515396264513No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2580.16279656739020695No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2550.1609035840484604No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2480.1564866229177183No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2430.153331650681474No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2410.15206966178697628No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2390.15080767289247854No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2340.14765270065623423No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2340.14765270065623423No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2310.14575971731448764No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2300.14512872286723877No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2270.14323573952549218No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2230.14071176173649672No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG2220.14008076728924784No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2220.14008076728924784No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2220.14008076728924784No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2200.13881877839475013No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGTCTCAG2190.13818778394750125No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2180.1375567895002524No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG2180.1375567895002524No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG2150.13566380615850582No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2140.13503281171125694No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2140.13503281171125694No Hit
GANTANACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2120.13377082281675923No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG2110.13313982836951035No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2110.13313982836951035No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2100.13250883392226148No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG2090.13187783947501264No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2090.13187783947501264No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2080.13124684502776374No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2080.13124684502776374No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2070.1306158505805149No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.12935386168601715No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2050.12935386168601715No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2020.12746087834427056No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2010.1268298838970217No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1990.12556789500252397No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1970.12430590610802625No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1920.12115093387178194No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1910.12051993942453307No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGTCTCAG1910.12051993942453307No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1900.11988894497728421No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1890.11925795053003535No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1890.11925795053003535No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1880.11862695608278646No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1880.11862695608278646No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1870.1179959616355376No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA1860.11736496718828873No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1850.11673397274103987No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1850.11673397274103987No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1820.11484098939929328No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1810.11420999495204442No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1790.1129480060575467No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGTCTCAG1780.11231701161029783No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1780.11231701161029783No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1770.11168601716304896No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCANGCTCTCGTGCATCAG1760.1110550227158001No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1760.1110550227158001No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1750.11042402826855124No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG1740.10979303382130237No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1730.10916203937405351No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1730.10916203937405351No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1730.10916203937405351No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1730.10916203937405351No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1720.10853104492680465No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1720.10853104492680465No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1680.10600706713780918No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1680.10600706713780918No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1670.10537607269056032No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.10537607269056032No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1650.10411408379606259No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA1640.10348308934881373No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1640.10348308934881373No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1630.10285209490156486No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1630.10285209490156486No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1620.102221100454316No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1620.102221100454316No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1600.10095911155981827No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1600.10095911155981827No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1590.1003281171125694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)