FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52986
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11482.166610047937191No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11452.16094817498962No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8091.5268184048616618No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6921.3060053599063903No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5421.0229117125278375No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5411.021024421545314No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5341.007813384667648No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4840.9134488355414638No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4340.8190842864152794No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4290.8096478315026612No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4050.7643528479220926No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4030.7605782659570453No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4000.7549163930094742No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3860.7284943192541425No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3570.6737628807609557No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3520.6643264258483373No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3060.5775110406522478No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2980.5624127127920583No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2860.539765221001774No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2680.5057939833163477No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2630.4963575284037293No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2520.47559732759596873No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2520.47559732759596873No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2400.45294983580568454No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2400.45294983580568454No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2390.45106254482316077No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2330.4397387989280187No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2190.41331672517268714No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2110.39821839731249764No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC2090.39444381534745027No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2040.3850073604348318No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2000.3774581965047371No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1970.3717963235571661No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1960.36990903257464236No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1930.36424715962707127No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1890.3566979956969766No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1890.3566979956969766No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1880.35481070471445286No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1870.3529234137319292No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1840.34726154078435817No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1800.33971237685426336No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1710.3227267580115502No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1690.31895217604650283No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1650.31140301211640814No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1610.30385384818631334No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1520.28686822934360023No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1510.2849809383610765No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1480.27931906541350543No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.269882610500887No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1410.2661080285358397No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1390.2623334465707923No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1380.2604461555882686No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1370.2585588646057449No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.2547842826406975No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1350.2547842826406975No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1330.2510097006756502No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1320.2491224096931265No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1310.2472351187106028No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1310.2472351187106028No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1290.2434605367455554No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.24157324576303177No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG1250.23591137281546068No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1230.2321367908504133No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.2321367908504133No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG1170.22081304495527118No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1160.21892575397274755No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1140.21515117200770015No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1140.21515117200770015No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1110.20948929906012909No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1100.20760200807760537No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1070.20194013513003434No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1040.19627826218246328No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1010.19061638923489224No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1000.18872909825236855No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1000.18872909825236855No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG980.18495451628732118No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG970.1830672253047975No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG960.1811799343222738No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA950.17929264333975012No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.17740535235722643No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG920.17363077039217908No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG900.16985618842713168No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG890.16796889744460802No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG880.16608160646208434No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA870.16419431547956062No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG860.16230702449703696No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG860.16230702449703696No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.16230702449703696No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.16041973351451327No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG850.16041973351451327No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG840.1585324425319896No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA840.1585324425319896No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA840.1585324425319896No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA830.1566451515494659No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA830.1566451515494659No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG820.1547578605669422No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC820.1547578605669422No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG820.1547578605669422No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG800.15098327860189484No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG800.15098327860189484No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG790.14909598761937115No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG770.14532140565432378No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG760.14343411467180012No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG750.1415468236892764No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC750.1415468236892764No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC730.13777224172422906No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG730.13777224172422906No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG720.13588495074170537No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA720.13588495074170537No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG720.13588495074170537No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG710.13399765975918165No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC710.13399765975918165No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG700.132110368776658No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG690.1302230777941343No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA690.1302230777941343No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG680.12833578681161062No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC680.12833578681161062No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA660.12456120484656324No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG650.12267391386403956No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG650.12267391386403956No Hit
GANTANTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG640.12078662288151588No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG640.12078662288151588No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG640.12078662288151588No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG630.11889933189899218No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG620.1170120409164685No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA620.1170120409164685No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA610.11512474993394482No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG610.11512474993394482No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG610.11512474993394482No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG610.11512474993394482No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG610.11512474993394482No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA590.11135016796889743No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA590.11135016796889743No Hit
GANTANAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG590.11135016796889743No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG590.11135016796889743No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG580.10946287698637377No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG580.10946287698637377No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG580.10946287698637377No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG580.10946287698637377No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA570.10757558600385007No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG570.10757558600385007No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA570.10757558600385007No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG570.10757558600385007No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG570.10757558600385007No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG570.10757558600385007No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC560.10568829502132639No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG560.10568829502132639No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG560.10568829502132639No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA560.10568829502132639No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG560.10568829502132639No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA550.10380100403880269No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG550.10380100403880269No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG550.10380100403880269No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG550.10380100403880269No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG550.10380100403880269No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG540.10191371305627903No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG530.10002642207375533No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG530.10002642207375533No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG530.10002642207375533No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG530.10002642207375533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)