FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128463
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC93167.251893541330967No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC44583.470259919198524No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA39673.088048698847139No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT32382.520570125250072No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA29002.257459346270911No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC26942.0971018892599425No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC22911.7833928835540196No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC22111.7211181429672355No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20211.573215634073624No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC20141.5677665942722807No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA19781.539742961008228No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT16291.268069405198384No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15461.203459361839596No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14181.1038197769007418No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC13631.061005892747328No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA13471.0485509446299712No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA13211.0283116539392667No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12941.007293928991227No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA11870.9240014634564038No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC11510.895977830192351No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGGAAGCCTGAC11180.8702894997003028No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC10910.8492717747522632No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10700.8329246553482326No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA10300.8017872850548406No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC10260.7986735480255015No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9480.7379556759533874No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC9330.7262791620933654No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9150.7122673454613391No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC8820.6865790149692907No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA8490.6608906844772425No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC7820.6087355892358111No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7800.6071787207211415No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7040.548017717163697No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGGAAGCCTGAC6650.5176587811276399No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA6550.5098744385542919No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6460.5028685302382787No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA6360.4950841876649308No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA6280.4888567136062524No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC6240.48574297657691323No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA6140.4779586340035652No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT6130.47718019974623044No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC6100.474844896974226No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5910.4600546460848649No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC5750.4475996979675082No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC5720.44526439519550376No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5660.4405937896514949No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5170.40245051104208995No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC5140.40011520827008557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5080.3954446027260768No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA5010.3899955629247332No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4950.38532495738072436No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC4810.37442687777803724No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4530.352630718572663No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC4470.34796011302865415No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT4430.34484637599931495No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4130.32149334827927106No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4080.3176011769925971No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT4080.3176011769925971No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4020.3129305714485883No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3980.3098168344192491No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT3950.30748153164724473No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3820.29736188630189236No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3520.2740088585818485No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC3480.2708951215525093No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC3430.26700295026583526No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3430.26700295026583526No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC3330.25921860769248734No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC3310.25766173917781776No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3300.25688330492048295No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC3300.25688330492048295No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3270.25454800214847856No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC3270.25454800214847856No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3230.25143426511913936No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC3200.24909896234713494No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA3100.24131461977378701No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA3090.2405361855164522No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3050.237422448487113No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGGAAGCCTGAC3050.237422448487113No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2950.22963810591376507No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC2950.22963810591376507No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2940.22885967165643026No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2930.22808123739909547No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2910.22652436888442587No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2780.21640472353907353No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC2720.21173411799506472No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2570.20005760413504278No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2560.199279169877708No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT2520.1961654328483688No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGGAAGCCTGAC2490.1938301300763644No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGAGAGACTGAA2450.19071639304702523No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA2410.18760265601768603No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2370.18448891898834685No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA2350.18293205047367725No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC2320.18059674770167286No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2290.17826144492966844No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2240.17436927364299448No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA2220.17281240512832488No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT2090.1626927597829725No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2070.16113589126830294No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAAAGCCTGAA2050.15957902275363334No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2040.15880058849629855No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1980.15412998295228977No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1970.153351548694955No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1970.153351548694955No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1930.15023781166561578No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGGCGCAAGCCTGAC1930.15023781166561578No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1920.14945937740828097No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1920.14945937740828097No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1860.14478877186427222No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT1840.14323190334960262No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1830.1424534690922678No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1820.14167503483493302No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC1790.13933973206292863No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1770.13778286354825903No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1710.13311225800425025No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1700.13233382374691544No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1680.13077695523224586No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1640.12766321820290666No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1580.1229926126588979No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1580.1229926126588979No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1570.1222141784015631No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA1550.12065730988689351No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC1520.1183220071148891No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1520.1183220071148891No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1490.11598670434288473No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC1480.11520827008554993No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1450.11287296731354553No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1440.11209453305621074No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1440.11209453305621074No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1430.11131609879887594No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1410.10975923028420634No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1410.10975923028420634No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA1410.10975923028420634No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGGCAATGGGGGAAACCCTGAC1390.10820236176953676No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1390.10820236176953676No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1370.10664549325486716No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1330.10353175622552796No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGAGAGACTGAA1290.1004180191961888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)