FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128463
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23431.8238714649354288No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21751.693094509703183No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA19381.5086055907148364No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA17591.3692658586519075No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16661.2968714727197714No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG16601.2922008671757628No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15811.2307045608463136No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG15581.2128005729276132No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC10060.7831048628788057No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9230.7184948195200175No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC8780.6834652779399516No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8150.6344239197278594No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7680.5978375096331239No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG7210.5612510995383885No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7010.5456824143916925No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG6860.5340059005316705No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6850.5332274662743358No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6750.5254431237009878No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6510.5067607015249528No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6360.4950841876649308No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6230.48496454231957836No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6220.4841861080622436No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG6180.4810723710329044No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG6080.4732880284595564No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6060.47173115994488685No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6020.46861742291554764No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5800.4514918692541821No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5780.44993500073951254No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5670.4413722239088298No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5570.4335878813354818No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG5540.43125257856347743No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG5450.4242466702474642No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5410.4211329332181251No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5020.39077399718206796No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5000.3892171286673984No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4990.38843869441006357No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA4800.3736484435207024No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA4750.3697562722340285No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG4630.3604150611460109No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4520.35185228431532817No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4240.33005612510995386No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4050.3152658742205927No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG4010.3121521371912535No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3920.30514622887524034No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3840.29891875481656194No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3720.28957754372854444No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3700.2880206752138748No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3640.28335006966986603No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3560.2771225956111877No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3480.2708951215525093No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3410.2654460817511657No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3390.2638892132364961No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3360.2615539104644917No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3360.2615539104644917No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3210.24987739660446975No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3140.2444283568031262No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3100.24131461977378701No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3030.23586557997244342No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3020.2350871457151086No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2910.22652436888442587No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2770.21562628928173871No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2730.21251255225239954No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2670.20784194670839073No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2660.20706351245105592No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2640.20550664393638635No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2640.20550664393638635No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2640.20550664393638635No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2620.20394977542171674No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2620.20394977542171674No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2610.20317134116438199No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2590.20161447264971238No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2580.2008360383923776No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2570.20005760413504278No Hit
GANTANTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2490.1938301300763644No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2450.19071639304702523No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2450.19071639304702523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2430.18915952453235563No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2420.1883810902750208No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2270.17670457641499887No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2190.1704771023563205No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2160.1681417995843161No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2150.16736336532698132No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2140.1665849310696465No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2140.1665849310696465No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2140.1665849310696465No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2130.16580649681231172No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2120.16502806255497693No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2090.1626927597829725No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2050.15957902275363334No Hit
GANTANAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2030.15802215423896374No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2030.15802215423896374No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2020.15724371998162895No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2010.15646528572429416No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1980.15412998295228977No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1950.15179468018028539No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1920.14945937740828097No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG1890.1471240746362766No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1870.145567206121607No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1830.1424534690922678No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1830.1424534690922678No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1830.1424534690922678No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1820.14167503483493302No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1770.13778286354825903No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1750.13622599503358945No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1690.13155538948958068No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1680.13077695523224586No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1670.12999852097491107No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1600.12454948117356747No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1600.12454948117356747No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1580.1229926126588979No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1540.1198788756295587No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1540.1198788756295587No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGTGCCTCAG1530.1191004413722239No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1510.1175435728575543No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG1510.1175435728575543No Hit
GANTANAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG1490.11598670434288473No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1490.11598670434288473No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGTGCCTCAG1450.11287296731354553No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCCTCAG1440.11209453305621074No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTGCCCTGGCTTTCGCACATGAG1440.11209453305621074No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG1430.11131609879887594No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1420.11053766454154114No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1400.10898079602687157No Hit
GANTANTGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG1370.10664549325486716No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1350.10508862474019756No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1320.10275332196819317No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1320.10275332196819317No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.10197488771085837No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGTGCCTCAG1310.10197488771085837No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG1310.10197488771085837No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1300.10119645345352359No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGTGCCTCAG1300.10119645345352359No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1290.1004180191961888No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGTGCCTCAG1290.1004180191961888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)