FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187120
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT123756.613403163745191No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA122116.525758871312528No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA94945.073749465583583No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA58033.101218469431381No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT56953.0435014963659683No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA46062.4615220179563915No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA45242.41769987174006No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA42872.2910431808465153No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC36861.96985891406584No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT35541.8993159469858916No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA33121.7699871740059856No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA31151.6647071398033348No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT29711.5877511757161182No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA27201.453612654980761No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA24241.2954253954681487No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA23831.273514322359983No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA21891.1698375374091492No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA20831.1131893971782814No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC18741.0014963659683624No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA16780.8967507481829842No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15360.8208636169303121No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA13220.7064985036340317No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13020.6958101752885849No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13000.6947413424540402No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA12650.6760367678495084No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA12530.6696237708422403No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGTGAGAGCCTGAA12470.6664172723386063No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA12280.6562633604104318No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA12000.6412997007268063No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA11760.6284737067122702No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11490.614044463445917No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC11320.6049593843522872No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC10540.563274903805045No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA10470.5595339888841385No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA9900.5290722530996151No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC9620.5141085934159897No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9600.5130397605814451No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9440.5044890979050877No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC9030.48257802479692175No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT8720.4660111158614793No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT8460.4521162890123985No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA7920.42325780247969225No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA7910.4227233860624198No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC7710.412035057716973No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA7410.39600256519880295No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7360.3933304831124412No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7010.37462590850790933No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6920.3698161607524583No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA6850.36607524583155193No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA6770.36179991449337323No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6670.35645575032064986No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC6440.34416417272338606No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6310.33721675929884565No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGTGAGAGCCTGAA6170.3297349294570329No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6000.32064985036340315No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT5560.29713552800342025No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5470.29232578024796924No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5450.29125694741342456No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5200.2778965369816161No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4880.26079521162890124No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4740.2533133817870885No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC4740.2533133817870885No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC4650.24850363403163744No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA4580.24476271911073108No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4560.2436938862761864No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4550.24315946985891407No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4540.24262505344164173No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA4540.24262505344164173No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4360.23300555793073965No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4200.22445489525438223No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4180.22338606241983755No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4160.22231722958529287No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4020.21483539974348015No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3970.21216331765711843No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3960.21162890123984612No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3860.2062847370671227No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT3770.20147498931167165No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA3730.1993373236425823No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC3670.19613082513894828No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3590.19185549380076958No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3540.18918341171440786No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3360.17956391620350579No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGTGAGAGCCTGAA3320.17742625053441644No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3270.17475416844805472No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3230.17261650277896537No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGTGAGAGCCTGAA3200.17101325352714836No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3170.16941000427533132No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT3090.16513467293715262No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA3080.16460025651988028No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3040.16246259085079093No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3000.16032492518170158No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2950.1576528430953399No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA2850.15230867892261649No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA2810.15017101325352714No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2710.14482684908080376No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC2610.13948268490808038No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2590.1384138520735357No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2510.13413852073535698No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC2320.12398460880718257No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2320.12398460880718257No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC2310.12345019238991023No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT2300.12291577597263789No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT2300.12291577597263789No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2270.12131252672082087No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2260.12077811030354853No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2230.11917486105173151No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2230.11917486105173151No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2230.11917486105173151No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC2220.11864044463445918No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA2170.11596836254809749No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAAAGCCTGAA2150.11489952971355281No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2100.11222744762719111No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2090.11169303120991877No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2090.11169303120991877No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2030.10848653270628475No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT1970.1052800342026507No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1950.10421120136810602No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1940.10367678495083368No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1940.10367678495083368No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1910.10207353569901667No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC1900.10153911928174432No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1880.10047028644719964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)