FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139390
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG53163.813759954085659No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG52463.763541143554057No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA48533.481598392998063No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA47953.439988521414736No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG33002.3674582107755215No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG31222.239758949709448No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22101.5854795896405767No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA19651.40971375277997No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18061.295645311715331No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17801.276992610660736No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA16761.2023818064423561No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA16601.1909032211779897No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA14451.0366597316880695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13740.9857235095774446No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13220.9484181074682546No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12870.9233087022024535No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11660.8365019011406843No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10860.7791089748188536No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10610.7611736853432814No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10600.7604562737642585No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9870.708085228495588No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8920.6399311284884138No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8640.619843604275773No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8330.5976038453260636No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8150.5846904369036516No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7980.5724944400602626No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7650.5488198579525073No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7370.5287323337398665No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG7010.5029055168950427No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG6910.4957314011048138No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6700.4806657579453332No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6550.46990458425998993No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5910.42399024320252526No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5630.40390271898988445No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5220.3744888442499462No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5120.36731472845971735No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4950.3551187316163283No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG4860.3486620274051223No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4850.3479446158260994No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4800.344357557930985No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4610.33072673792955015No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4560.3271396800344357No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4540.32570485687639No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4410.3163785063490925No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4340.3113566252959323No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4090.2934213358203602No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3880.2783556926608795No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3620.2597029916062845No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3600.2582681684482388No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3520.25252887581605565No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3350.2403328789726666No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3300.23674582107755218No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3280.23531099791950644No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3270.23459358634048355No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3080.2209627663390487No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG2910.20876676949565967No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2900.20804935791663678No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA2870.20589712317956813No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2840.20374488844249944No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG2840.20374488844249944No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2780.19944041896836218No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG2780.19944041896836218No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA2770.19872300738933926No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2750.1972881842312935No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG2730.19585336107324774No Hit
GANTANTGGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG2680.1922663031781333No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2670.1915488915991104No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2660.19083148002008754No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2640.18939665686204174No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG2620.18796183370399597No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG2610.1872444221249731No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG2600.1865270105459502No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG2550.18293995265083576No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA2520.18078771791376713No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG2510.18007030633474425No Hit
GANTANTGGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG2510.18007030633474425No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2500.17935289475572136No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG2500.17935289475572136No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA2490.17863548317669847No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA2480.1779180715976756No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2480.1779180715976756No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA2460.17648324843962981No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG2450.17576583686060693No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2450.17576583686060693No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG2450.17576583686060693No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA2430.17433101370256116No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG2410.17289619054451538No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2400.1721787789654925No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG2400.1721787789654925No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA2390.17146136738646964No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2380.17074395580744672No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA2380.17074395580744672No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2340.1678743094913552No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA2330.1671568979123323No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2330.1671568979123323No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA2320.16643948633330943No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG2320.16643948633330943No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2290.16428725159624077No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG2260.1621350168591721No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA2230.15998278212210346No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2230.15998278212210346No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA2220.15926537054308057No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA2210.15854795896405766No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA2200.1578305473850348No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA2160.15496090106894325No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA2150.15424348948992037No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCTTCAG2150.15424348948992037No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2140.15352607791089748No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG2130.1528086663318746No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA2100.15065643159480593No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2090.14993902001578305No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2070.1485041968577373No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2060.1477867852787144No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.13989525790946267No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1930.1384604347514169No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1920.137743023172394No Hit
GANTANTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1920.137743023172394No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1900.13630820001434824No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1870.13415596527727958No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1850.1327211421192338No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1840.13200373054021092No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCTTCAG1840.13200373054021092No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1790.12841667264509649No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1780.1276992610660736No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1770.1269818494870507No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1760.12626443790802783No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1740.12482961474998205No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1630.11693808738073032No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1600.11478585264366166No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1600.11478585264366166No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1590.11406844106463879No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1590.11406844106463879No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1580.11335102948561589No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1570.11263361790659301No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1560.11191620632757013No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1540.11048138316952436No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1530.10976397159050146No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1500.10761173685343282No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1470.10545950211636415No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1470.10545950211636415No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1450.10402467895831839No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1450.10402467895831839No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1440.10330726737929549No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1440.10330726737929549No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1440.10330726737929549No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1440.10330726737929549No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1430.10258985580027262No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1420.10187244422124972No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1420.10187244422124972No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1400.10043762106320396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)