FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104985
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG48934.660665809401343No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG41253.9291327332476067No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25952.4717816830975856No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20661.9679001762156498No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG19541.8612182692765635No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG17211.6392818021622138No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG14711.4011525456017526No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11111.058246416154689No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10591.0087155307901128No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9640.9182264132971377No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9460.9010811068247846No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8640.8229747106729532No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8070.7686812401771681No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7560.7201028718388341No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA7360.7010525313139973No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7350.7001000142877554No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6920.6591417821593561No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6750.6429489927132447No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6740.6419964756870029No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG6600.6286612373196171No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5940.5657951135876553No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5850.5572224603514788No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG5650.5381721198266419No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5630.5362670857741583No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG5170.4924513025670334No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5140.48959375148830786No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5040.48006858122588947No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5020.47816354717340576No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4830.46006572367481063No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4430.42196504262513695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG4270.4067247702052674No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG4260.40577225317902554No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3950.37624422536552843No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3830.36481402105062627No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3640.34671619755203126No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3580.3410010953945802No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3520.3352859932371291No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3430.32671334000095253No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG3340.3181406867647759No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3330.3171881697385341No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3320.31623565271229226No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG3270.311473067581083No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3170.30194789731866456No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3150.30004286326618085No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3110.2962327951612135No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3060.2914702100300043No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2980.28385007382006955No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2910.27718245463637664No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2880.2743249035576511No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG2860.2724198695051674No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2840.27051483545268373No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG2660.25336952898033055No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG2660.25336952898033055No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2620.24955946087536315No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2590.24670190979663764No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG2470.23527170548173548No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG2430.23146163737676811No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG2430.23146163737676811No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2420.23050912035052626No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG2400.22860408629804257No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2390.22765156927180072No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2380.22669905224555886No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG2380.22669905224555886No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2370.22574653521931706No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG2360.2247940181930752No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG2350.22384150116683335No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG2320.22098395008810784No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG2290.2181263990093823No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG2250.21431633090441493No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG2250.21431633090441493No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2250.21431633090441493No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2240.21336381387817308No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG2240.21336381387817308No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG2220.2114587798256894No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2150.20479116064199648No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2140.20383864361575463No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2110.20098109253702912No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2100.20002857551078726No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG2060.19621850740581986No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG2050.19526599037957804No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2030.19336095632709435No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG2020.1924084393008525No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2010.19145592227461067No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG2000.1905034052483688No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1980.18859837119588513No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1980.18859837119588513No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1940.18478830309091776No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1930.1838357860646759No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG1920.18288326903843408No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1850.17621564985474114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1850.17621564985474114No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1850.17621564985474114No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1810.17240558174977377No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1800.17145306472353192No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1780.16954803067104823No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1780.16954803067104823No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1750.16669047959232272No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1750.16669047959232272No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1700.1619278944611135No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG1670.15907034338238796No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.15716530932990427No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG1630.15526027527742056No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG1590.15145020717245322No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCAG1530.14573510501500214No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1510.14383007096251846No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1430.1362099347525837No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1420.13525741772634187No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1410.1343049007001No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG1410.1343049007001No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1400.13335238367385818No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1400.13335238367385818No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1380.13144734962137447No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.13049483259513264No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG1340.1276372815164071No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1320.12573224746392342No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1300.12382721341143972No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1290.12287469638519789No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1270.1209696623327142No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG1270.1209696623327142No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1270.1209696623327142No Hit
GANTANTGGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG1270.1209696623327142No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1260.12001714530647237No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1250.11906462828023051No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG1250.11906462828023051No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1250.11906462828023051No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1250.11906462828023051No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1240.11811211125398867No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG1230.11715959422774681No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG1230.11715959422774681No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1230.11715959422774681No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1230.11715959422774681No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1230.11715959422774681No Hit
GANTANTGGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1230.11715959422774681No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1220.11620707720150499No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGCGCATCAG1220.11620707720150499No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1220.11620707720150499No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1210.11525456017526313No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG1210.11525456017526313No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1210.11525456017526313No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1190.11334952612277943No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG1180.1123970090965376No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1160.11049197504405392No Hit
GANTANTAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1150.10953945801781208No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG1150.10953945801781208No Hit
GANTANTAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1140.10858694099157022No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1110.1057293899128447No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1100.10477687288660284No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1100.10477687288660284No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1100.10477687288660284No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1090.10382435586036101No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1070.10191932180787731No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1070.10191932180787731No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCAG1070.10191932180787731No Hit
GANTANTAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1060.10096680478163546No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1060.10096680478163546No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1060.10096680478163546No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1060.10096680478163546No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1050.10001428775539363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)