FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133749
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA96147.188091125914959No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA79795.965652079641718No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41733.120023327277213No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA38012.841890406657246No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG33762.524131021540348No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA33762.524131021540348No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG32782.4508594456780988No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG26772.001510291665732No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA25951.940201422066707No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA20541.5357124165414322No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15971.1940276188980852No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG15621.1678591989472817No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15141.1319710801576086No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA13841.0347740917689106No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13030.9742128913113369No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12920.9659885307553702No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12850.9607548467652095No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG12070.9024366537319904No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11890.888978609185863No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11880.8882309400444115No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10780.8059873344847437No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10520.7865479368070041No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9830.734958766046849No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8380.6265467405363778No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8190.6123410268487989No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7250.5420601275523556No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7180.5368264435621949No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6150.45981652199268785No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6100.4560781762854301No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6060.45308749971962403No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5850.43738644774914204No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5810.43439577118333594No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5530.4134610352226933No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA5240.391778630120599No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5110.3820589312817292No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA4950.3700962250185048No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA4910.3671055484526987No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4850.3626195336039896No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA4840.36187186446253805No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA4820.360376526179635No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA4790.35813351875528043No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4790.35813351875528043No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4740.3543951730480228No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA4600.34392780506770143No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA4510.3371987827946377No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4410.3297220913801225No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA4320.3229930691070587No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4220.3155163776925435No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4120.30803968627802825No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4110.3072920171365767No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4100.3065443479951252No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4000.2990676565806099No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3970.29682464915625534No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA3930.29383397259044924No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA3930.29383397259044924No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3920.2930863034489977No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA3910.2923386343075462No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA3880.2900956268831916No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA3860.2886002886002886No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3810.28486194289303096No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA3800.28411427375157944No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA3770.28187126632722487No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3630.27140389834690354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3490.2609365303665822No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA3480.2601888612251307No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA3440.2571981846593246No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3190.23850645612303645No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2990.22355307329400592No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2760.20635668304062088No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2690.20112299905046016No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2640.19738465334320254No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2510.18766495450433274No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2420.18093593223126903No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2390.17869292480691445No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2270.16972089510949614No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2200.16448721111933548No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.16074886541207783No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG2130.1592535271291748No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG2120.15850585798772326No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2080.15551518142191717No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2080.15551518142191717No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG2060.15401984313901412No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2050.1532721739975626No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2050.1532721739975626No Hit
GANTANTAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA2020.15102916657320803No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG1990.14878615914885343No Hit
GANTANTAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1980.1480384900074019No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1970.14729082086595038No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG1930.1443001443001443No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1910.14280480601724124No Hit
GANTANTAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA1910.14280480601724124No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA1910.14280480601724124No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1890.1413094677343382No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1890.1413094677343382No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG1890.1413094677343382No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1880.14056179859288667No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1830.13682345288562905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1830.13682345288562905No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG1820.13607578374417753No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1800.13458044546127448No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.13308510717837144No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.13308510717837144No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1770.1323374380369199No Hit
GANTANTAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA1770.1323374380369199No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG1760.1315897688954684No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG1750.13084209975401687No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.12934676147111382No Hit
GANTANTAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA1720.1285990923296623No Hit
GANTANTAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA1700.12710375404675925No Hit
GANTANTAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA1680.12560841576385617No Hit
GANTANTAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA1680.12560841576385617No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA1670.12486074662240465No Hit
GANTANTGGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG1670.12486074662240465No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG1650.1233654083395016No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1640.12261773919805008No Hit
GANTANCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1630.12187007005659856No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1620.12112240091514703No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG1600.11962706263224399No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1580.11813172434934094No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1580.11813172434934094No Hit
GANTANTGGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1560.11663638606643789No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1550.11588871692498637No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1540.11514104778353484No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1530.11439337864208332No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1520.11364570950063177No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1500.11215037121772872No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG1470.10990736379337415No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG1470.10990736379337415No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1470.10990736379337415No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1450.1084120255104711No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1430.10691668722756806No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1430.10691668722756806No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1430.10691668722756806No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1430.10691668722756806No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAA1420.10616901808611653No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1400.10467367980321349No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG1360.10168300323740737No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1360.10168300323740737No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1360.10168300323740737No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1350.10093533409595584No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1340.10018766495450432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)