FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110004
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG26672.424457292462092No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG24102.190829424384568No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG20681.8799316388494964No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG18901.7181193411148685No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG15621.4199483655139813No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA15461.4054034398749136No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12021.0926875386349586No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG11641.0581433402421732No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11191.017235736882295No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10250.9317842987527726No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10020.910875968146613No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG9990.9081487945892877No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG9670.8790589433111524No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9610.873604596196502No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9050.822697356459765No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8320.7563361332315188No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8070.7336096869204756No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7140.6490673066433947No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG7000.6363404967092106No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6770.6154321661030508No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6580.5981600669066579No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG6500.590887604087124No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG6330.5754336205956146No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5970.5427075379077124No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG5840.5308897858259699No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG5460.49634558743318424No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5360.48725500890876694No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5260.47816443038434964No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5240.4763463146794662No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5090.4627104468928403No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4990.453619868368423No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG4770.4336205956147049No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG4650.42271190138540415No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4510.40998509145122No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG4150.3772590087633177No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4060.3690774880913421No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3890.35362350459983277No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3810.3463510417802989No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3800.3454419839278572No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3790.3445329260754154No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3790.3445329260754154No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3770.342714810370532No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3680.33453328969855645No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3640.3308970582887895No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3640.3308970582887895No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3540.3218064797643722No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3490.31726119050216356No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3430.3118068433875132No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG3340.3036253227155376No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG3310.30089814915821245No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3280.29817097560088723No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG3280.29817097560088723No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3200.29089851278135337No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3070.27908076069961096No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2890.2627177193556598No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2860.25999054579833464No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2810.25544525653612593No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2750.24999090942147562No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2750.24999090942147562No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAG2670.24271844660194172No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2540.2309006945201993No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2520.2290825788153158No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAG2470.22453728955310714No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2460.22362823170066545No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2450.2227191738482237No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2440.22181011599578196No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2350.2136285953238064No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2340.21271953747136468No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2300.20908330606159778No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2260.20544707465183087No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2230.20271990109450563No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2210.2009017853896222No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG2190.19908366968473873No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2170.1972655539798553No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2160.19635649612741354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2120.19272026471764664No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAG2120.19272026471764664No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2110.1918112068652049No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG2100.19090214901276317No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2100.19090214901276317No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG2070.188174975455438No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG2060.18726591760299627No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2060.18726591760299627No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2050.18635685975055452No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG1980.17999345478346243No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAG1970.17908439693102068No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCAG1960.17817533907857896No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1960.17817533907857896No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1890.17181193411148685No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1880.17090287625904513No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1860.16908476055416166No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1850.16817570270171994No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1760.1599941820297444No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1760.1599941820297444No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1710.15544889276753573No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1690.15363077706265227No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1690.15363077706265227No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1680.15272171921021055No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCAG1670.1518126613577688No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG1650.14999454565288534No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1640.14908548780044362No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1630.1481764299480019No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1590.14454019853823497No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCAG1560.14181302498090978No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1550.14090396712846806No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1540.1399949092760263No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1520.13817679357114285No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1510.13726773571870113No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG1510.13726773571870113No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1510.13726773571870113No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTANNNANANTTTCGAGCCTCAG1480.13454056216137594No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1480.13454056216137594No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1460.13272244645649248No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAG1450.13181338860405076No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1450.13181338860405076No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1430.12999527289916732No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1430.12999527289916732No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1420.12908621504672557No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCAG1420.12908621504672557No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1390.1263590414894004No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAG1380.12544998363695864No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1370.12454092578451693No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1340.12181375222719174No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1340.12181375222719174No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACANTTTCGAGCCTCAG1330.12090469437475002No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTACCNACACTTTCGAGCCTCAG1330.12090469437475002No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1330.12090469437475002No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAG1320.11999563652230827No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1300.11817752081742482No Hit
GANTANTAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAG1270.11545034726009964No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNG1260.1145412894076579No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1260.1145412894076579No Hit
GANTANTAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAG1240.11272317370277445No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAG1240.11272317370277445No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1240.11272317370277445No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNG1230.11181411585033273No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAG1220.11090505799789098No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1220.11090505799789098No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1200.10908694229300753No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTANCNANANTTTCGAGCCTCNG1200.10908694229300753No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCANACTTTCGAGCCTCAG1200.10908694229300753No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1190.10817788444056581No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1180.10726882658812406No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAG1180.10726882658812406No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG1180.10726882658812406No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCANGNTTTCGAGCTTCAG1170.10635976873568234No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1160.10545071088324061No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAG1150.10454165303079889No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTANNNANANTTTCGAGCCTCAG1150.10454165303079889No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG1130.10272353732591544No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1130.10272353732591544No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1120.10181447947347369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)