FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86478
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG78759.106362311801846No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG69308.013598834385624No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG31623.656421286338722No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG29393.398552232937857No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG25462.944101389948889No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG17542.02826152316196No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG12521.4477670621429728No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11481.3275052614537801No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA11001.271999814981845No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10701.2373089109368856No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10681.234996184000555No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9911.145956196951826No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6250.7227271676033211No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6060.7007562617081801No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6010.6949744443673536No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA5170.5978399130414672No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG4750.549272647378524No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4610.5330835588242097No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4600.5319271953560443No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4290.49607992784291954No Hit
GANTANAAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4230.48914174703392777No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG4200.48567265662943176No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG4190.4845162931612665No Hit
GANTANAAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG4160.4810472027567705No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4160.4810472027567705No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG4100.47410902194777865No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG4100.47410902194777865No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA4070.47063993154328265No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG4060.4694835680751174No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG4040.46717084113878676No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG4030.4660144776706215No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG4020.4648581142024561No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG4010.4637017507342908No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG3930.4544508429889683No Hit
GANTANAAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG3880.44866902564814176No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3870.4475126621799764No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG3680.4255417562848355No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG3670.4243853928166701No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3610.4174472120076782No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3550.41050903119868637No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG3450.3989453965170332No Hit
GANTANTGGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG3350.3873817618353801No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG3220.37234903674923103No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG3140.3630981290039085No Hit
GANTANTGGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG3060.353847221258586No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3040.35153449432225536No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2970.3434399500450982No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2890.3341890422997757No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2880.33303267883161036No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2710.31337449987279997No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2380.2752145054233447No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2320.2682763246143528No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2310.26711996114618747No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2220.25671268993269963No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2090.24167996484655055No Hit
GANTANCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1980.2289599666967321No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1910.2208654224195749No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA1860.21508360507874832No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA1830.2116145146742524No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1800.20814542426975646No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1790.20698906080159116No Hit
GANTANTAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG1750.20236360692892993No Hit
GANTANTAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG1680.19426906265177268No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG1670.1931126991836074No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1640.18964360877911146No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG1620.18733088184278082No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1620.18733088184278082No Hit
GANTANCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1590.18386179143828488No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA1530.176923610629293No Hit
GANTANTAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG1530.176923610629293No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG1510.17461088369296238No Hit
GANTANTAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG1490.17229815675663174No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG1480.17114179328846643No Hit
GANTANTAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG1480.17114179328846643No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1450.1676727028839705No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1440.16651633941580518No Hit
GANTANTAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG1380.1595781586068133No Hit
GANTANTAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG1350.15610906820231737No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1340.15495270473415204No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1340.15495270473415204No Hit
GANTANTAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1270.14685816045699485No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1220.14107634311616826No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1200.13876361617983765No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1070.12373089109368857No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG1050.12141816415735794No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAA1040.12026180068919262No Hit
GANTANCAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG1020.11794907375286201No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.11563634681653137No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG960.11101089294387012No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG920.10638543907120887No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG910.10522907560304355No Hit
GANTANCAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG900.10407271213487823No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG890.10291634866671291No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA890.10291634866671291No Hit
GANTANCAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG890.10291634866671291No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCGCACTTTCGAGCTTCAG890.10291634866671291No Hit
GANTANCAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG880.10175998519854759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)