FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200654
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2014710.040667018848366No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG170168.480269518673936No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG79403.957060412451284No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG69133.445234084543543No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG58892.9349028676228732No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG43412.16342559829358No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG28771.4338114366023105No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA28091.3999222542286722No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG26961.34360640704895No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG26221.306727002701167No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG25941.2927726334884926No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA25041.2479193038763243No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG15870.7909137121612329No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG13820.6887477947112941No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12320.6139922453576804No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11240.5601682498230784No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG11140.5551845465328377No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG11110.5536894355457653No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10700.5332562520557776No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG10230.5098328465916453No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG9940.4953801070499466No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9820.4893996631016576No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9730.4849143301404408No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG9720.48441595981141666No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9700.4834192191533685No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA9660.4814257378372721No Hit
GANTANTGGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG9270.46198929500533253No Hit
GANTANAAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9260.4614909246763084No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9240.46049418401826026No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG9240.46049418401826026No Hit
GANTANAAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG9030.4500284071087544No Hit
GANTANAAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG8950.4460414444765616No Hit
GANTANTGGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG8810.43906425987022435No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG8750.43607403789607985No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG8730.4350772972380316No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8720.43457892690900757No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG8630.43009359394779073No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG8560.42660500164462206No Hit
GANTANTGGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8500.4236147796704775No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG8320.41464411374804394No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8220.40966041045780294No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG8160.4066701884836584No Hit
GANTANTGGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG8060.40168648519341754No Hit
GANTANTGGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG7530.3752728577551407No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6510.32443908419468337No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6310.3144716776142016No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5790.2885564205049488No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5450.2716118293181297No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA5180.25815583043447926No Hit
GANTANCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5100.25416886780228654No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4980.24818842385399745No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4810.23971612826058789No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4630.2307454623381542No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4620.23024709200913013No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4580.2282536106930338No Hit
GANTANTAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG4390.21878457444157604No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4350.21679109312547967No Hit
GANTANTAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG4210.2098139085191424No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4080.2033350942418292No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4080.2033350942418292No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG4030.20084324259670877No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3960.19735465029354013No Hit
GANTANTAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG3960.19735465029354013No Hit
GANTANTAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG3930.19585953930646785No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3880.1933676876613474No Hit
GANTANCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3810.18987909535817876No Hit
GANTANTAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3650.1819051700937933No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3610.17991168877769692No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG3550.1769214668035524No Hit
GANTANTAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG3490.17393124482940783No Hit
GANTANTAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG3490.17393124482940783No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3490.17393124482940783No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3290.16396383824892602No Hit
GANTANTAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG3260.16246872726185374No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3060.1525013206813719No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG3030.15100620969429962No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2830.14103880311381783No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2650.13206813719138416No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2640.13156976686236008No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2530.12608769324309507No Hit
GANTANCAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG2520.125589322914071No Hit
GANTANCAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG2460.12259910093992644No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2430.12110398995285417No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2390.1191105086367578No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2390.1191105086367578No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCAG2390.1191105086367578No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2360.11761539764968552No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACACTTTCGAGCTTCAG2340.11661865699163734No Hit
GANTANCAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG2310.11512354600456506No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2240.11163495370139644No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2200.10964147238530007No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2190.10914310205627598No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2170.1081463613982278No Hit
GANTANCAGGGTATCTAATNCTGTTCGATANCNGNANTTTCGAGCTTCNG2150.10714962074017961No Hit
GANTANCAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG2140.10665125041115552No Hit
GANTANCAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG2110.10515613942408324No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG2100.10465776909505915No Hit
GANTANCAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2100.10465776909505915No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2090.10415939876603506No Hit
GANTANCAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG2020.10067080646286643No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2010.10017243613384234No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCGCACTTTCGAGCTTCAG2010.10017243613384234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)