FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147926
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG38192.5816962535321717No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA35212.380244176142125No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34052.301826588970161No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA31362.1199789083731053No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG23531.590660194962346No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG20871.4108405554128416No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG20591.3919121723023673No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG19631.3270148587807418No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15851.0714816867893406No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG15761.0653975636466881No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG14510.9808958533320713No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA13580.9180265808579965No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12870.8700296093992942No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA12130.8200045968930412No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11900.8044562821951518No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11500.7774157348944742No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11240.759839379149034No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10120.6841258467071373No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG9340.6313967794708165No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9020.6097643416302746No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG8920.6030042048051053No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8120.5489231102037505No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7820.5286426997282425No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7500.5070102618877006No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG7370.49822208401498047No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7190.48605383772967564No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6800.45968930411151526No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6620.44752105782621043No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6480.4380568662709733No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG6400.43264875681083786No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG6330.4279166610332193No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6200.41912848316049917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6140.41507240106539756No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG6100.4123683463353298No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5880.3974960453199573No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG5860.39614401795492343No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5790.39141192217730486No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5710.3860038127171694No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5560.3758636074794154No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5330.3603152927815259No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5280.35693522436894126No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5200.3515271149088057No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG5190.3508511012262888No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5180.3501750875437719No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4980.3366548138934332No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4930.3332747454808485No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4910.3319227181158147No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4430.2994740613550018No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4390.2967700066249341No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4260.28798182875221395No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4260.28798182875221395No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4120.27851763719697686No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4060.27446155510187525No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3830.2589132404039858No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3810.2575612130389519No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3710.25080107621378256No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3580.24201289834106243No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3380.22849262469072373No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3310.22376052891310522No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3300.22308451523058828No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3250.2197044468180036No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3220.2176764057704528No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG3210.21700039208793587No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3200.21632437840541893No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3180.2149723510403851No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3170.21429633735786813No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3130.21159228262780042No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2770.18725579005719076No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2760.18657977637467382No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2730.18455173532712302No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2690.18184768059705528No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2640.17846761218447063No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2630.17779159850195367No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2620.17711558481943676No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2610.17643957113691983No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2490.1683274069467166No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2480.16765139326419967No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2400.1622432838040642No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2310.15615916066141178No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2280.15413111961386097No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2270.15345510593134407No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2270.15345510593134407No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2260.1527790922488271No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2250.1521030785663102No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2190.14804699647120856No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2170.14669496910617472No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA2150.14534294174114085No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2150.14534294174114085No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2150.14534294174114085No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG2150.14534294174114085No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2140.14466692805862388No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2130.14399091437610698No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2100.14196287332855617No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2060.13925881859848843No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2040.13790679123345456No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2010.13587875018590376No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1990.1345267228208699No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1980.13385070913835295No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG1980.13385070913835295No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1960.13249868177331908No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1950.13182266809080218No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANGNTTTCGAGCCTCAG1940.1311466544082852No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1920.12979462704325134No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA1900.1284425996782175No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG1870.12641455863066667No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1870.12641455863066667No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTCCCNACGCTTTCGAGCCTCAG1860.12573854494814976No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA1850.12506253126563283No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1840.12438651758311589No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1830.12371050390059894No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1830.12371050390059894No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1820.12303449021808201No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG1800.12168246285304814No Hit
GANTANTGGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1800.12168246285304814No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1800.12168246285304814No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA1740.11762638075794654No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1740.11762638075794654No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.11695036707542959No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA1720.11627435339291267No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1700.11492232602787882No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANGNTTTCGAGCCTCAG1690.11424631234536187No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA1690.11424631234536187No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1690.11424631234536187No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGAGCCTCAG1680.11357029866284495No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA1670.112894284980328No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA1660.11221827129781106No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA1650.11154225761529414No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA1640.11086624393277719No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANGNTTTCGAGCCTCNG1640.11086624393277719No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1620.10951421656774332No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1610.1088382028852264No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1600.10816218920270947No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGAGCCTCAG1600.10816218920270947No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1590.10748617552019255No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1590.10748617552019255No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA1580.1068101618376756No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG1570.10613414815515865No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1560.10545813447264173No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1560.10545813447264173No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGAGCCTCNG1550.10478212079012479No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1550.10478212079012479No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1550.10478212079012479No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA1540.10410610710760787No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1540.10410610710760787No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1530.10343009342509092No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.10343009342509092No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1520.102754079742574No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA1520.102754079742574No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1510.10207806606005705No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCANGCTTTCGAGCCTCAG1510.10207806606005705No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA1500.10140205237754012No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA1500.10140205237754012No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1480.10005002501250625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)