FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135111
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1653612.238825854297577No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA74895.542849953001606No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA71285.275662233274863No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA47973.5504141039589667No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44883.3217132579878768No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC33372.4698211100502547No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA32102.3758243222239495No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA31142.304771632213513No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA20151.4913663580315444No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19081.4121722139574129No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT18541.3722050758265425No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC17611.303372782378933No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC16471.2189977129915404No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA15861.1738496495474091No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA15541.1501654195439306No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC14831.0976160342237122No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA13611.0073199073354502No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA13611.0073199073354502No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA13110.9703132979550148No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT12890.9540303898276233No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA12820.9488494645143623No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12740.9429284070134927No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC10270.760115756674142No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA9620.712007164479576No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8580.6350334169682705No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA8360.618750508840879No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT7860.5817438994604437No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC7740.5728623132091392No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC7240.5358557038287038No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA7210.5336353072658777No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7090.5247537210145733No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7020.5195727957013122No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC6770.5010694910110947No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6540.4840464506960943No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC6540.4840464506960943No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA6530.48330631850848566No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA6270.46406288163065923No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGAC6260.4633227494430505No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6170.4566615597545722No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT6160.45592142756696347No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5950.44037865162718065No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT5600.4144740250608759No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA5570.4122536284980497No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC5550.4107733641228324No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA4920.36414503630348377No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA4690.34712199598848353No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4680.3463818638008748No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4600.3404608063000052No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4260.31529631192130914No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA4140.30641472567000466No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA4090.3027140647319611No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4020.2975331394187002No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC3970.29383247848065663No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3960.2930923462930479No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3960.2930923462930479No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA3920.2901318175426131No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3790.2805100991036999No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3740.2768094381656564No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3700.27384890941522155No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3660.27088838066478677No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC3550.26274692660109095No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT3490.25830613347543874No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2930.21685873096935113No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2920.21611859878174242No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA2920.21611859878174242No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA2910.2153784665941337No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC2870.21241793784369886No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA2860.2116778056560902No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2820.20871727690565536No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC2800.20723701253043794No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA2770.2050166159676118No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2730.202056087217177No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2690.19909555846674218No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2670.19761529409152473No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC2580.1909541044030464No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC2560.18947384002782897No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2550.18873370784022028No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2510.18577317908978544No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2430.17985212158891578No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGAC2430.17985212158891578No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC2410.17837185721369836No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA2300.1702304031500026No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2190.16208894908630683No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2140.15838828814826328No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA2110.15616789158543717No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2110.15616789158543717No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGAC2050.15172709845978494No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1960.14506590877130654No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1930.14284551220848044No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1920.14210538002087172No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1850.13692445470761078No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1850.13692445470761078No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1810.13396392595717596No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1810.13396392595717596No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1760.1302632650191324No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1750.12952313283152372No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1750.12952313283152372No Hit
CCTANGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1700.12582247189348017No Hit
CCTANGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1690.1250823397058715No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1690.1250823397058715No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGGCAATGGAGGAAACTCTGAC1670.12360207533065407No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1660.12286194314304534No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1640.12138167876782793No Hit
CCTANGGGAGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC1620.11990141439261051No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1600.1184211500173931No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC1600.1184211500173931No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1550.11472048907934958No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1540.11398035689174087No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT1530.11324022470413217No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1520.11250009251652344No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1520.11250009251652344No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1480.10953956376608862No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1470.10879943157847992No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1430.1058389028280451No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA1370.10139810970239285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)