FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135111
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG32282.389146701600906No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA31192.308472293151557No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA28082.078291182805249No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG26851.9872549237293782No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14821.0968759020361036No Hit
GACTACTGGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG13060.9666126370169713No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG13030.9643922404541452No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA12340.9133231195091444No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11780.8718757170030567No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11590.8578132054384914No Hit
GACTACAAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG11220.8304283144969692No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11030.8163658029324037No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA10750.79564210167936No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9850.7290302047945763No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9300.6883229344760975No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA9050.6698196297858797No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA9040.669079497598271No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA7520.5565794050817476No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA7440.550658347580878No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6920.5121714738252252No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6850.5069905485119642No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6700.49588856569783357No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6110.45222076662891997No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5840.43223719756348483No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5750.42557600787500643No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5580.4129937606856584No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5530.4092930997476149No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5450.4033720422467453No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5420.40115164568391914No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5280.39078979505739725No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5280.39078979505739725No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG5240.3878292663069624No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4980.36858582942913604No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4860.35970424317783156No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4830.35748384661500543No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4730.3500825247389184No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4660.34490159942565746No Hit
GACTACTAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG4610.3412009384876139No Hit
GANTANTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4600.3404608063000052No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG4560.33750027754957035No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4540.3360200131743529No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4530.3352798809867442No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4480.33157922004870066No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4470.33083908786109195No Hit
GANTANTGGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG4470.33083908786109195No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4400.32565816254783103No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4290.31751670848413527No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG4290.31751670848413527No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA4240.3138160475460917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4110.30419432910717853No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC4110.30419432910717853No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4040.2990134037939176No Hit
GANTANAAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG3760.2782897025408738No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3750.27754957035326505No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3720.275329173790439No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3620.2679278519143519No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3620.2679278519143519No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3600.2664475875391345No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3540.2620067944134823No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG3480.25756600128783No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3460.2560857369126126No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3350.24794428284891679No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG3140.232401506909134No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3110.23018111034630784No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3070.227220581595873No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC3060.22648044940826434No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2950.21833899534456852No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2940.21759886315695984No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2690.19909555846674218No Hit
GANTANCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2680.19835542627913344No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2650.19613502971630734No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2620.1939146331534812No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2610.19317450096587252No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2550.18873370784022028No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2450.1813323859641332No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2370.17541132846326354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2370.17541132846326354No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2310.1709705353376113No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2290.16949027096239389No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2280.16875013877478517No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2280.16875013877478517No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2270.16801000658717646No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2270.16801000658717646No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG2200.16282908127391552No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2130.15764815596065457No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2130.15764815596065457No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2130.15764815596065457No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2060.15246723064739362No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2040.1509869662721762No Hit
GACTACAGGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG2030.15024683408456752No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2030.15024683408456752No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2020.1495067018969588No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2010.1487665697093501No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2000.14802643752174138No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1980.14654617314652396No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1970.14580604095891528No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1930.14284551220848044No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1920.14210538002087172No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1900.1406251156456543No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1890.13988498345804562No Hit
GACTACCAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG1880.1391448512704369No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1880.1391448512704369No Hit
GANTANAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1860.1376645868952195No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1850.13692445470761078No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1820.13470405814478464No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1770.13100339720674115No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1760.1302632650191324No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1740.128783000643915No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1740.128783000643915No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1730.1280428684563063No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1730.1280428684563063No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1720.1273027362686976No Hit
GANTANAAGGGTATCTAATNNTGTTCGATANNNGNANTTTCGAGCTTCAG1710.12656260408108888No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG1710.12656260408108888No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1700.12582247189348017No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1690.1250823397058715No Hit
GANTANTGGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG1680.12434220751826276No Hit
GANTANAAGGGTATCTAATCCTGTTCGNTNNCCGNANTTTCGAGCTTCAG1680.12434220751826276No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1680.12434220751826276No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATGAA1630.12064154658021924No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATGAA1630.12064154658021924No Hit
GANTANAAGGGTATNTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1630.12064154658021924No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1620.11990141439261051No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1620.11990141439261051No Hit
GANTANAAGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG1600.1184211500173931No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1590.1176810178297844No Hit
GANTANACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1590.1176810178297844No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCGNACTTTCGAGCTTCAG1590.1176810178297844No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCNG1580.1169408856421757No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1570.116200753454567No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1550.11472048907934958No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1550.11472048907934958No Hit
GACTACTCGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG1550.11472048907934958No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1530.11324022470413217No Hit
GANTANTAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG1520.11250009251652344No Hit
GANTANTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1510.11175996032891475No Hit
GANTANTGGGGTATNTAATNNTGTTCGNTANNNGNANTTTCGAGCTTCAG1510.11175996032891475No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1510.11175996032891475No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATGAA1500.11101982814130604No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1500.11101982814130604No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1490.11027969595369734No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATGNA1490.11027969595369734No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1460.10805929939087121No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1460.10805929939087121No Hit
GANTANAAGGGTATNTAATCNTGTTCGATACCNGCACTTTCGAGCTTCAG1450.10731916720326251No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1450.10731916720326251No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1440.1065790350156538No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCGCANTTTCGAGCTTCAG1430.1058389028280451No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATGAA1430.1058389028280451No Hit
GACTACACGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCAG1430.1058389028280451No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG1420.10509877064043638No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1410.10435863845282768No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA1380.10213824189000155No Hit
GANTANAAGGGTATCTAATCNTGTTCGATANNNGNANTTTCGAGCTTCAG1370.10139810970239285No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1370.10139810970239285No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1370.10139810970239285No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA1370.10139810970239285No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1360.10065797751478414No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1360.10065797751478414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)