FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002463584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158836
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA52053.276964919791483No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG46322.9162154675262535No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA41732.6272381575965147No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG38482.422624593920774No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG33632.1172781988969755No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG28211.7760457327054322No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25331.5947266362789294No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22651.4259991437709336No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG22311.4045934171094714No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG20441.2868619204714298No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA19681.2390138255811025No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG18621.1722783248130146No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA18181.144576796192299No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG16491.0381777430809136No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA14990.9437407136921101No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13330.8392304011685009No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG13250.8341937596010979No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG13000.818454254702964No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG12100.7617920370696819No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10990.6919086353219672No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10790.6793170314034601No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10390.6541338235664459No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10020.6308393563172077No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9950.6264322949457302No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9480.5968420257372384No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9050.569770077312448No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG8140.5124782794832407No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7790.49044297262585307No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7680.48351759047067416No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7490.47155556674809235No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG7250.45644564204588384No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7170.451409000478481No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG7040.4432244579314513No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7020.44196529753960057No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG6660.41930041048628774No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG6600.4155229293107356No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6410.40356090558815383No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6380.4016721650003777No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5680.3576015512856028No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5610.3531944899141253No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5330.3355662444282153No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG5270.3317887632526631No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5210.328011282077111No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4730.2977914326726938No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4560.2870885693419628No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4420.2782744465990078No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4370.275126545619381No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4220.26568284268050063No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4200.26442368228864993No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3910.24616585660681456No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3830.2411292150394117No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3820.24049963484348638No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3730.23483341308015815No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3680.23168551210053134No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3630.2285376111209046No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3610.22727845072905384No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3530.22224180916165104No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3520.22161222896572566No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3420.2153164270064721No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA3320.20902062504721852No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3280.2065023042635171No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3260.20524314387166637No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG3140.1976881815205621No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2910.1832078370142789No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2880.18131909642650282No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2850.17943035583872674No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2850.17943035583872674No Hit
GANTANTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2820.17754161525095066No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA2810.1769120350550253No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2810.1769120350550253No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG2780.17502329446724924No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA2780.17502329446724924No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG2770.17439371427132389No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2720.17124581329169708No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA2710.17061623309577173No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA2680.16872749250799568No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG2660.16746833211614495No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2650.1668387519202196No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA2620.16495001133244352No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA2620.16495001133244352No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA2610.16432043113651817No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA2580.1624316905487421No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCCTCAA2570.16180211035281675No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2550.16054294996096602No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAA2530.15928378956911532No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAG2530.15928378956911532No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2510.1580246291772646No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA2480.15613588858948854No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG2460.15487672819763781No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG2460.15487672819763781No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2440.15361756780578711No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2440.15361756780578711No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA2420.1523584074139364No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2410.15172882721801104No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG2390.1504696668261603No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG2360.14858092623838423No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCCTCAA2340.14732176584653353No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2330.14669218565060818No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCAA2320.14606260545468283No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2320.14606260545468283No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2290.14417386486690673No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG2220.13976680349542925No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2210.1391372232995039No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAG2180.13724848271172782No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.13535974212395174No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2130.13410058173210102No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2120.1334710015361757No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG2110.13284142134025032No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2110.13284142134025032No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2110.13284142134025032No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCCTCAA2100.13221184114432497No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATCAG2080.13095268075247424No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATCAG2070.1303231005565489No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2050.1290639401646982No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG2030.12780477977284746No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2030.12780477977284746No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCAG1990.12528645898914603No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1990.12528645898914603No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATCAG1950.1227681382054446No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1950.1227681382054446No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1940.12213855800951925No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1920.12087939761766854No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1890.11899065702989248No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1860.11710191644211639No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG1860.11710191644211639No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1860.11710191644211639No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA1850.11647233624619104No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATCNG1850.11647233624619104No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCCTCAA1830.11521317585434032No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCCTCNA1830.11521317585434032No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1830.11521317585434032No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATCNG1820.11458359565841497No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1810.11395401546248961No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTANNNANANTTTCGAGCCTCAG1800.11332443526656426No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1800.11332443526656426No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1800.11332443526656426No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1780.11206527487471354No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1760.11080611448286283No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1750.11017653428693747No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1730.10891737389508677No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG1720.10828779369916139No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCANACTTTCGAGCCTCAG1700.10702863330731067No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1680.10576947291545996No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATCAG1680.10576947291545996No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAG1680.10576947291545996No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1670.10513989271953461No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG1670.10513989271953461No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACANTTTCGAGCCTCAG1660.10451031252360926No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTANNNANANTTTCGAGCCTCAG1660.10451031252360926No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATCAG1650.1038807323276839No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.10325115213175855No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTACCNACACTTTCGAGCCTCAG1630.10262157193583318No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCCTCAG1620.10199199173990783No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG1610.10136241154398247No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1600.10073283134805712No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1600.10073283134805712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)