FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153138
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA47763.118755632174901No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA40552.6479384607347622No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA29471.9244080502553254No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA25191.6449215740051457No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA18641.21720278441667No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18141.184552495135107No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG17931.1708393736368503No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG17751.1590852694954876No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA17001.1101098355731431No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA15351.0023638809439852No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14880.9716726090193158No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA13380.8737217411746268No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG13310.8691507006752079No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11760.7679348039023626No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11750.7672817981167314No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10750.7019812195536052No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA10520.6869620864840863No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10380.6778200054852486No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA9410.6144784442790163No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8550.5583199467147278No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8540.5576669409290966No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA8030.5243636458619023No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG7850.5126095417205396No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7240.4727761887970327No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7230.4721231830114015No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7110.4642871135838263No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6870.4486149747286761No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6660.4349018532304196No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6560.42837179537410697No Hit
GANTANTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG6390.4172706970183756No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6310.41204665073332547No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6200.4048635870913816No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA6060.39572150609254403No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6030.39376248873565023No Hit
GANTANAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG5980.3904974598074939No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5960.3891914482362314No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA5940.3878854366649689No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5620.36698925152476847No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5560.3630712168109809No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5480.35784717052593085No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA5450.3558881531690371No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG5350.3493580953127245No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5230.34152202588514935No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5050.3297679217437866No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4640.30299468453290496No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4530.2958116208909611No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4470.29189358617717354No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4360.28471052253522966No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG4340.2834045109639671No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA4260.27818046467891705No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4230.27622144732202325No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4100.2677323721088169No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG4080.26642636053755436No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4020.2625083258237668No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3970.2592432968956105No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3890.2540192506105604No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3890.2540192506105604No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3860.25206023325366667No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3710.24226514646919772No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3670.23965312332667266No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG3670.23965312332667266No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3590.23442907704162258No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG3500.22855202497094126No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3460.2259400018284162No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA3340.21810393240084108No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3260.212879886115791No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3210.2096148571876347No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3170.20700283404510963No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3140.20504381668821586No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG3060.1998197704031658No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2970.19394271833248444No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2970.19394271833248444No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2930.19133069518995938No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2860.18675965469054057No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2770.1808826026198592No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2710.17696456790607165No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA2590.1691284984784965No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2580.16847549269286527No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.167822486907234No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2560.16716948112160274No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2530.16521046376470896No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.16390445219344643No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2450.1599864174796589No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG2430.15868040590839635No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2400.15672138855150258No Hit
GANTANAAGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2400.15672138855150258No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2390.15606838276587132No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATGAA2380.15541537698024005No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2360.15410936540897752No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATGAA2360.15410936540897752No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGNA2340.15280335383771498No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG2330.15215034805208374No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGAA2330.15215034805208374No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA2320.15149734226645248No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2320.15149734226645248No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATGAA2300.15019133069518995No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2300.15019133069518995No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG2270.14823231333829617No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2270.14823231333829617No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC2220.14496728441013987No Hit
GANTANTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTGAG2200.14366127283887734No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2190.1430082670532461No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA2180.14235526126761483No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2180.14235526126761483No Hit
GANTANAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG2160.1410492496963523No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2130.13909023233945852No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG2110.13778422076819602No Hit
GANTANTGGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATGAA2110.13778422076819602No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2100.13713121498256475No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2090.1364782091969335No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCATGAA2070.13517219762567095No Hit
GANTANAAGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATGAA2060.13451919184003971No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2060.13451919184003971No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2040.13321318026877718No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2030.13256017448314592No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2030.13256017448314592No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2020.13190716869751468No Hit
GANTANTGGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATGNA2010.1312541629118834No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1980.1292951455549896No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1980.1292951455549896No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1970.12864213976935837No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGNA1950.12733612819809584No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1940.12668312241246457No Hit
GANTANTGGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCATGAA1920.12537711084120207No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1880.12276508769867701No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGAGCCTCAA1870.12211208191304575No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1870.12211208191304575No Hit
GANTANAAGGGTATCTAATNCTGTTTGATANCNANANTTTCGAGCATGNA1850.12080607034178321No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1850.12080607034178321No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1820.11884705298488944No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCANACTTTCGAGCATGAA1810.11819404719925818No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACANTTTCGAGCATGAA1780.1162350298423644No Hit
GANTANAAGGGTATCTAATCNTGTTTGATANNNANANTTTCGAGCCTCNA1770.11558202405673314No Hit
GANTANTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG1730.1129700009142081No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1680.1097049719860518No Hit
GANTANTGGGGTATNTAATCCTGTTTGATACCCACACTTTCGAGCATGAA1680.1097049719860518No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTANNNANANTTTCGAGCATGAA1670.10905196620042054No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1660.10839896041478927No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1660.10839896041478927No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1650.10774595462915801No Hit
GANTANTGGGGTATNTAATCNTGTTTGATACCNACACTTTCGAGCATGAA1620.10578693727226424No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG1610.10513393148663297No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG1590.10382791991537044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)