FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002463588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002463588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70961
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA19592.7606713546877866No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA16192.2815349276363075No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13351.8813150885697776No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11251.5853778836262173No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10951.5431011400628514No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG9621.3556742435985965No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9571.3486281196713688No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8071.1372444018545398No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7311.0301433181606798No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6820.961091303673849No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6150.8666732430489987No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5400.7609813841405842No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5340.7525260354279111No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5340.7525260354279111No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5280.7440706867152379No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4910.6919293696537535No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4870.6862924705119714No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA4500.6341511534504869No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4320.6087851073124674No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4260.6003297585997942No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4240.5975113090289033No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4200.591874409887121No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4190.5904651851016756No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4050.5707360381054383No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3660.5157762714730627No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3540.49886557404771636No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3530.4974563492622709No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG3440.4847733261932611No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3380.47631797748058796No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG3260.4594072800552416No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3160.44531503220078633No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC3080.43404123391722216No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA3010.4241766604191034No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC2980.41994898606276687No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2920.4114936373500937No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2900.4086751877792027No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2880.4058567382083116No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2670.37626301771395554No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2630.3706261185721735No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2630.3706261185721735No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2530.3565338707177182No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2430.3424416228632629No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA2350.33116782457969873No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2270.3198940262961345No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2250.31707557672524345No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2110.2973464297290061No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2070.291709530587224No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2060.29030030580177846No Hit
GANTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2060.29030030580177846No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1970.2776172827327687No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1960.27620805794732317No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1940.2733896083764321No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1880.264934259663759No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1870.2635250348783134No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1820.25647891095108577No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1800.25366046138019477No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1790.25225123659474924No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1740.24520511266752162No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1670.2353405391694029No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1660.2339313143839574No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1640.23111286481306634No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1640.23111286481306634No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1640.23111286481306634No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1620.22829441524217525No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.22124829131494766No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1490.20997449303138344No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1460.20574681867504685No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1460.20574681867504685No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1450.20433759388960135No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.2015191443187103No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1410.1987006947478192No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1390.19588224517692815No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.19306379560603712No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG1370.19306379560603712No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1350.19024534603514606No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1310.18460844689336395No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.18460844689336395No Hit
GANTANTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1310.18460844689336395No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1310.18460844689336395No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATCAG1280.1803807725370274No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG1260.17756232296613633No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1230.17333464860979975No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1210.1705161990389087No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1210.1705161990389087No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1210.1705161990389087No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1200.1691069742534632No Hit
GANTANAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG1170.1648792998971266No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1170.1648792998971266No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.1592424007553445No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1120.15783317596989896No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1120.15783317596989896No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANANTTTCGTGCCTCAA1110.15642395118445343No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1090.15360550161356237No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1090.15360550161356237No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1090.15360550161356237No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1080.15219627682811684No Hit
GANTANAAGGGTATNTAATNNTGTTTGNTCNNNANANTTTCGTGCCTCAA1060.14937782725722581No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1050.14796860247178026No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCANACTTTCGTGCCTCAA1050.14796860247178026No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1040.14655937768633473No Hit
GANTANAAGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGTGCCTCAA1020.1437409281154437No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1020.1437409281154437No Hit
GANTANTGGGGTATNTAATCNTGTTTGCTCCCNACACTTTCGTGCCTCAA1020.1437409281154437No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1000.14092247854455264No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANANTTTCGTGCCTCAA980.13810402897366159No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG980.13810402897366159No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG960.13528557940277053No Hit
GANTANTGGGGTATCTAATCCTGTTTGNTNNCCANANTTTCGTGCCTCAA950.133876354617325No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG950.133876354617325No Hit
GANTANAAGGGTATNTAATCNTGTTTGCTCCCNACACTTTCGTGCCTCAA920.1296486802609884No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC920.1296486802609884No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG910.12823945547554289No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA900.12683023069009738No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA900.12683023069009738No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.12683023069009738No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA900.12683023069009738No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCANACTTTCGTGCCTCAA900.12683023069009738No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCNCNANANTTTCGTGCCTCNA890.12542100590465186No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA890.12542100590465186No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.12401178111920631No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA880.12401178111920631No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG870.12260255633376081No Hit
GANTANTGGGGTATCTAATCNTGTTTGCTCNNNANANTTTCGTGCCTCAA870.12260255633376081No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG860.12119333154831527No Hit
GANTANAAGGGTATNTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG860.12119333154831527No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG850.11978410676286974No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG840.11837488197742423No Hit
GANTANAAGGGTATCTAATNNTGTTTGATANNNANANTTTCGAGCATCAG840.11837488197742423No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG840.11837488197742423No Hit
GANTANTGGGGTATNTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA830.1169656571919787No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG830.1169656571919787No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACANTTTCGTGCCTCAA820.11555643240653317No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG820.11555643240653317No Hit
GANTANAAGGGTATCTAATNNTGTTTGCTCNNNANGNTTTCGCGCCTCAG810.11414720762108763No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCNG810.11414720762108763No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC810.11414720762108763No Hit
GANTANTGGGGTATCTAATNCTGTTTGCTCNCNANANTTTCGTGCCTCNA790.11132875805019658No Hit
GANTANTGGGGTATNTAATNNTGTTTGNTCNNNANANTTTCGTGCCTCAA780.10991953326475105No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG780.10991953326475105No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC770.10851030847930554No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG770.10851030847930554No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAC750.10569185890841448No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA750.10569185890841448No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG750.10569185890841448No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANGNTTTCGCGCCTCAG750.10569185890841448No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG750.10569185890841448No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.10428263412296897No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA740.10428263412296897No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCNNNANANTTTCGTGCCTCNA720.1014641845520779No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG720.1014641845520779No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG720.1014641845520779No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG710.10005495976663237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)