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screens in a set of Asian head and neck cancer cell lines to identify novel targets. @PG ID:SCS VN:1.18.66.3 PN:RTA DS:Controlling software on instrument @PG ID:basecalling PP:SCS VN:1.18.66.3 PN:RTA DS:Basecalling Package @PG ID:bambi PP:basecalling VN:0.10.1 CL:/nfs/gs01/software/pkg/bambi/0.10.1/bin/bambi i2b --intensity-dir=/nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities --basecalls-dir=/nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BaseCalls --lane=1 --platform-unit=180508_HS40_25799_A_HFW7VBCX2_1 --read-group-id=25799_1 --study-name="EGAS00001002204: CRISPR/Cas9 lethality screens in a set of Asian head and neck cancer cell lines to identify novel targets." --sample-alias=EGAN00001730531,EGAN00001730532,EGAN00001730533,EGAN00001730534,EGAN00001730535,EGAN00001730536 --threads=3 --output-file=- --compression-level=0 PN:bambi DS:Convert Illumina BCL to BAM or SAM file @PG ID:bamadapterfind PN:bamadapterfind CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamadapterfind level=0 PP:bambi VN:2.0.79 @PG ID:bambi.1 PN:bambi PP:bamadapterfind VN:0.10.1 CL:/nfs/gs01/software/pkg/bambi/0.10.1/bin/bambi decode --metrics-file /nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/25799_1.bam.tag_decode.metrics --barcode-file /nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/metadata_cache_25799/lane_1.taglist --compression-level 0 - @PG ID:bamcollate2 PN:bamcollate2 CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamcollate2 collate=2 level=0 PP:bambi.1 VN:2.0.79 @PG ID:bwa PN:bwa CL:/nfs/gs01/software/pkg/bwa/0.7.16a/bwa samse /nfs/gs01/repository/references/PhiX/Sanger-SNPs/all/bwa0_6/phix_unsnipped_short_no_N.fa /tmp/8vk_mdxIY8/alnphix_bwa_aln_out /tmp/6wnSt5UIGu/alnphix_simple_cat_out PP:bamcollate2 VN:0.7.16a-r1181 @PG ID:scramble PN:scramble CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -t 2 -0 -I sam -O bam PP:bwa VN:1.14.9 @PG ID:bam12auxmerge PN:bam12auxmerge CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=1 zztoname=0 clipreinsert=1 /tmp/OaVe3ZjI_J/simple_cat_out PP:scramble VN:2.0.79 @PG ID:scramble.1 PN:scramble PP:bam12auxmerge VN:1.14.9 CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -I bam -O cram -x -3 @PG ID:scramble.2 PN:scramble PP:scramble.1 VN:1.14.9 CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -I cram -O bam -0 @PG ID:spf PN:spatial_filter PP:scramble.2 DS:A program to apply a spatial filter VN:0.10.1 CL:/nfs/gs01/software/pkg/bambi/0.10.1/bin/bambi spatial_filter -c -t /nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/archive/qc/tileviz/25799_1 -F /dev/stdout /dev/stdin ; /nfs/gs01/software/pkg/bambi/0.10.1/bin/bambi spatial_filter -a --compression-level 0 -f -F /tmp/L0e2BBbeB7/tee_post_filter_creation:__APPLY_FILTER_OUT___out -l /nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/25799_1.bam.filter.stats /dev/stdin @PG ID:samtools PN:samtools PP:spf VN:1.6 CL:/nfs/gs01/software/pkg/samtools/1.6/bin/samtools split -f /nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/lane1/%!.bam - @PG ID:bamcollate2' PN:bamcollate2 CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamcollate2 collate=1 level=0 PP:samtools VN:2.0.79 @PG ID:bamreset PN:bamreset CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamreset resetaux=0 level=0 verbose=0 PP:bamcollate2' VN:2.0.79 @PG ID:bamadapterclip PN:bamadapterclip CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamadapterclip verbose=0 level=0 PP:bamreset VN:2.0.79 @PG ID:bwa' PN:bwa CL:/nfs/gs01/software/pkg/bwa/0.7.16a/bwa samse /nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/bwa0_6/hs37d5.fa /tmp/mTaoR3FYbJ/aln_alntgt_bwa_aln_out /tmp/2ZlMPYQ96x/aln_alntgt_simple_cat_out PP:bamadapterclip VN:0.7.16a-r1181 @PG ID:scramble' PN:scramble CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -0 -I sam -O bam PP:bwa' VN:1.14.9 @PG ID:samtools' PN:samtools CL:/nfs/gs01/software/pkg/samtools/1.6/bin/samtools reheader /tmp/8SeVxJVyMv/aln_postaln_alterSQ_headerSQfix_out /tmp/NJzxN_oT4F/aln_postaln_mbuffer_headerSQfix_out PP:scramble' VN:1.6 @PG ID:bam12split PN:bam12split CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bam12split verbose=0 level=0 PP:samtools' VN:2.0.79 @PG ID:bam12auxmerge' PN:bam12auxmerge CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/0IgeAeLZRD/aln_amp_bamadapterclip_pre_auxmerge_out PP:bam12split VN:2.0.79 @PG ID:bambi.2 PN:bambi PP:bam12auxmerge' VN:0.10.1 CL:/nfs/gs01/software/pkg/bambi/0.10.1/bin/bambi select --compression-level 0 --input /tmp/ALLLgzUUIb/aln_tee3_tee3:to_phix_aln_out,/tmp/iaOEwtlX_l/aln_bam12auxmerge_out --output /tmp/SL7oz9r8oj/alignment_filter:phix_bam_out_out,/tmp/H3R_WKiBxf/alignment_filter:target_bam_out_out -m 25799_1#4.bam_alignment_filter_metrics.json DS:Split alignments into different files @PG ID:bamsormadup PN:bamsormadup CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamsormadup threads=32 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=/nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/archive/lane1/bsfopt_25799_1#4.tmp PP:bambi.2 VN:2.0.79 @PG ID:bamstreamingmarkduplicates PN:bamstreamingmarkduplicates CL:/nfs/gs01/software/pkg/biobambam/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=/nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/archive/lane1/bmdfopt_25799_1#4.tmp M=/nfs/gs02/IL_seq_data/analysis/180508_HS40_25799_A_HFW7VBCX2/Data/Intensities/BAM_basecalls_20180508-151026/no_cal/archive/lane1/25799_1#4.markdups_metrics.txt PP:bamsormadup VN:2.0.79 @PG ID:scramble.3 PN:scramble PP:bamstreamingmarkduplicates VN:1.14.9 CL:/nfs/gs01/software/pkg/staden_io_lib/1.14.9/bin/scramble -t 15 -7 -I bam -O cram -r /nfs/gs01/repository/references/Homo_sapiens/1000Genomes_hs37d5/all/fasta/hs37d5.fa @CO TY:checksum ST:all PA:all HA:crc32prod CO:28365447 BS:1423cf0f NS:342e457b SQ:3e62b71b ST:BC,FI,QT,RT,TC:4d82d15b @CO TY:checksum ST:all PA:pass HA:crc32prod CO:28365447 BS:1423cf0f NS:342e457b SQ:3e62b71b ST:BC,FI,QT,RT,TC:4d82d15b @CO TY:checksum ST: PA:all HA:crc32prod CO:0 BS:1 NS:1 SQ:1 ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST: PA:pass HA:crc32prod CO:0 BS:1 NS:1 SQ:1 ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST:25799_1#4 PA:all HA:crc32prod CO:28365447 BS:1423cf0f NS:342e457b SQ:3e62b71b ST:BC,FI,QT,RT,TC:4d82d15b @CO TY:checksum ST:25799_1#4 PA:pass HA:crc32prod CO:28365447 BS:1423cf0f NS:342e457b SQ:3e62b71b ST:BC,FI,QT,RT,TC:4d82d15b