FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002466089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20702354
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA617380.29821729451636275No Hit
GCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCT587430.2837503406617431No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT585300.2827214721572242No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC541800.2617093688959236No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA527730.2549130403238202No Hit
ATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC522610.25243989161812225No Hit
AACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAAT500600.24180825040476073No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA499260.24116098101694136No Hit
TGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGG492580.2379342948149761No Hit
TGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA462460.22338522469473762No Hit
AGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTT456500.22050632502951112No Hit
GGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACT438280.21170539350259399No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA436640.2109132130578001No Hit
TGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCT435820.21051712283540316No Hit
TGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGA422340.20400578600868285No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT407070.1966298132086815No Hit
GCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGG400380.19339829663815042No Hit
ACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGG400310.1933644840581897No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAAT390090.1884278473839255No Hit
GCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGT352820.1704250637391284No Hit
GATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG338670.16359009221849843No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA330070.15943597525189648No Hit
GCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAA330030.1594166537776332No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG321350.1552238938625047No Hit
CGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC306460.14803147506800435No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG295010.14250070306014476No Hit
CAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA294610.1423074883175121No Hit
GGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGAT292050.14107091396466315No Hit
ACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGT283320.13685400220670557No Hit
TGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGG283170.13678154667821832No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT272850.13179660631829598No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC270240.13053588012261794No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC269030.12995140552615417No Hit
GGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG268000.1294538775638751No Hit
AGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGG262360.12672954969275474No Hit
CCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCG258240.12473943784363846No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT257660.12445927646682112No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA254100.12273966525739055No Hit
ATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAA247530.11956611310964926No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG244560.11813149364560185No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATT242910.11733448283224217No Hit
ATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTC238000.11496277186642641No Hit
ATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAG234580.11331078581691724No Hit
ATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC232010.11206938109550248No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC231420.1117843893501193No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG230430.11130618286210352No Hit
ATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAG230140.11116610217369483No Hit
ATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCAT229020.11062510089432341No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT225960.10914700811318365No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA224740.10855770314815408No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCC224620.10849973872536427No Hit
TGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA224310.10834999729982396No Hit
TGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGT218810.10569329458862504No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGT217130.10488179266956793No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA216670.10465959571554037No Hit
TGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT216570.1046112920298822No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG214890.10379979011082509No Hit
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA212840.10280956455483276No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT211250.10204153595286797No Hit
GACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGC208500.1007131845972685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)