FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002466616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192955
Sequences flagged as poor quality0
Sequence length289
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTAACAGAGA151497.851053354409059No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA150957.823067554611179No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA82674.284418646834754No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA80064.149153947811666No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA79454.117540359151097No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGTTGATAGTCA75803.928377082739499No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACTGATA74693.8708507164883006No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATCACAGAAA56522.929180378844808No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATGGACAGTCA55412.87165401259361No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA55092.8550698349356067No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA53992.798061724236221No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA42822.219170272861548No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATTCACGGTTA42762.2160607395506724No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACATTGA38812.011349796584696No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA38071.9729988857505636No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA37851.9615972636106864No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCACCA37231.9294654193983054No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA30171.5635769998186104No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA28781.4915394781166593No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA26531.3749319789588246No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA26491.3728589567515743No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACCGTCA23801.233448213313985No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTGACCATTA22441.1629654582674718No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGGATGATTA19981.0354745925215725No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGAACAATAA19591.015262626000881No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGGGTGACCACAA18870.977948226270374No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA18560.9618823041641834No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTCACCACCA18450.9561814930942448No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTGACCAGAA17260.8945090824285455No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTCTTACAGTTA17240.8934725713249204No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA16520.8561581715944132No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACTGTCA15290.7924127387214634No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCAGCACGGACA14330.7426602057474541No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA14050.7281490502967013No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA13390.6939441838770698No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCCACCTTTA13160.68202430618538No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA12850.6659583840791895No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTGACCTGAA12600.6530019952838745No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAGACTTGTA11790.611023295587054No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA11490.595475629032676No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA11430.5923660957218004No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGTTTTACTTGTA10810.5602342515094193No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATGACTTGGA10790.5591977404057942No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCCACTTTTA10350.5363944961260397No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATGATGGTGA10020.519292062916224No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAACAGTCA9750.5052991630172838No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA9090.4710942965976523No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA8510.44103547459252157No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTGTGACCAACA8450.43792594128164597No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGATA7700.39905677489570107No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA7080.36692493068331994No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGACTTGACTCTCA7000.36277888626881916No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGAACTCACTGATA6740.34930424192169157No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAGACAGTCA5970.3093985644321215No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA5290.27415718690886476No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGTTGTTGCCAGGGA5250.27208416470161434No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTTACTGTCA4560.23632453162654504No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA4510.23373325386748206No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA4260.22077686507216707No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAAACCTTCA3710.19227280972247415No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTTATAATTA3480.18035293203078437No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACTGATA3390.175688632064471No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTCACAGTGA3280.1699878209945324No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT3210.16636003213184422Illumina Paired End PCR Primer 2 (100% over 50bp)
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTGGACAGCAA3110.16117747661371823No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAAGACAGGTGGAGA2990.15495840999196703No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA2870.14873934337021585No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTCAAGTCCTGGGGCT2770.14355678785208986No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGTCCAAGCATAAA2740.14200202119665206No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGCACTTGGA2580.1337099323676505No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGTCCAAGCATAAA2570.1331916768158379No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGTGGTAAAACAATCA2440.12645435464227409No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA2410.1248995879868363No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGCCTTCACCACCA2400.12438133243502371No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA2180.11297971029514654No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA2100.10883366588064573No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGAATAACGGTGA2030.10520587701795756No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA1990.10313285481070716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)