FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002466620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234720
Sequences flagged as poor quality0
Sequence length289
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTGACCAGAA3106313.23406612133606No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTAACAGAGA157736.719921608725289No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGTTTTACTTGTA116794.975715746421268No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA106104.5202794819359235No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA87243.716768916155419No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA61972.640167007498296No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACTGTCA61732.62994205862304No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGGATGATTA57472.448449216087253No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA45691.9465746421267895No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA41891.7846796182685754No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTATGTGGCTTCAA41231.7565610088616224No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA40561.7280163599182004No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGTTGATAGTCA36051.5358725289706885No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA35011.491564417177914No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATGATGGTGA32251.3739775051124745No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTCTTACAGTTA31601.3462849352419906No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA29941.275562372188139No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCAGCACGGACA29111.2402010906612133No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA27311.1635139740967961No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCACCA26921.1468984321745057No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGACTTGACTCTCA26481.1281526925698706No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTGACCAGCA25691.0944955691888207No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATTCACGGTTA25661.0932174505794137No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA25331.0791581458759372No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAA23030.981169052488071No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA22260.9483640081799591No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA22050.9394171779141104No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGAACAATAA21340.9091683708248125No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA21210.9036298568507158No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTGACCTGAA20410.8695466939331971No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA20200.8605998636673483No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTGACTCTCA19730.840576005453306No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAAACCTTCA19330.8235344239945467No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGGAGAATATGAA19090.8133094751192911No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA18280.7788002726653034No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTTTCTCCTCCAAAC18220.7762440354464895No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTTATAATTA17170.731509884117246No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAGACTTGTA15810.6735685071574642No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTAACAAATA14230.6062542603953647No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA13810.5883605998636674No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA13480.5743012951601909No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA13110.5585378323108385No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTCACCACCA12400.5282890252215405No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATCACAGAAA12390.5278629856850715No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTAAGATCCACCTTTA11520.49079754601227No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA11420.4865371506475801No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGAATAACGGTGA11190.47673824130879344No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCCACTTTTA10990.4682174505794137No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTACTTGGCTGGACA10680.4550102249488753No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA9920.4226312201772325No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTGGACAGCAA9400.4004771642808453No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA9030.38471370143149286No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCCTGCCAGAACCCGT8620.3672460804362645No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCCACCTTTA8320.35446489434219497No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGATTCACTGTGA8190.34892638036809814No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA8050.34296182685753235No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACCGTCA7650.325920245398773No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAAAAAAGATAAGGG7290.31058282208588955No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA6930.2952453987730061No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTTATAATTA6930.2952453987730061No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCCTAACTGCTA6870.2926891615541922No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGAACTCACTGATA6400.27266530334015No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAAAAAAGATAAGGG6350.27053510565780503No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAACAGTCA6270.26712678936605316No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGTTTTACTTGTAAAGTTGTC6040.25732788002726653No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGTGGTAAAACAATCA6010.25604976141785957No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTGTACAGCCA5880.2505112474437628No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTTTTACTGATA5830.24838104976141784No Hit
CAGCTGGTACACGGCAGGGTCAGGCTCCCGTACTCTGCGTTGATACCACT5670.2415644171779141Clontech SMART CDS Primer II A (95% over 22bp)
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA5650.24071233810497614No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGGGTGACCACAA5620.2394342194955692No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGCCAGCCC5440.2317655078391275No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGTATTCTGCCACC5360.2283571915473756No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA4970.2117416496250852No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA4590.1955521472392638No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA4520.19256987048398091No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTCCC4320.18404907975460122No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACTGATA4260.18149284253578732No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTCCCATCTTCATGG4250.18106680299931835No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCCCCGTACTCT4250.18106680299931835No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGTCTACATCGTA4150.1768064076346285No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGAGTCCTATCCAG4150.1768064076346285No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA3690.15720858895705522No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA3620.15422631220177233No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACTGTGA3620.15422631220177233No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATGACTTGGA3460.1474096796182686No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA3380.1440013633265167No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTGACCAAGT3320.1414451261077028No Hit
CAGCTGGTACACGGCAGGGTCAGGATTCTGGATATTGGGTATGATGGTGA3250.1384628493524199No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGATA3060.1303680981595092No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGCACTTGGA2950.1256816632583504No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTTTCACAGATA2900.12355146557600546No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA2770.11801295160190867No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTCAAGTCCTGGGGCT2720.11588275391956375No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTAACTCCCTTCTTG2500.10650988411724609No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTAACTAGCA2380.10139740967961827No Hit
CAGCTGGTACACGGCAGGTCAGGGTTCTGGATATTTGGGATGACTTGGAG2380.10139740967961827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)