FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002466622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288830
Sequences flagged as poor quality0
Sequence length289
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATCACAGAAA10090134.93439047190389No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA5047517.47567773430738No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA4228014.638368590520376No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA119044.121455527472908No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGAACTCACTGATA41881.4499878821452066No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACCGTCA40321.3959768722085657No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA31551.0923380535262956No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTCACAGTGA30481.0552920403005228No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC30271.0480213274244365Illumina Paired End PCR Primer 2 (100% over 49bp)
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTAACAGAGA29991.0383270435896548No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTCACCACCA27540.9535020600353149No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAGACAGTCA26890.9309974725617145No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGGATGATTA22690.7855832150399888No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA20780.7194543503098709No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA20160.6979884361042829No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA18040.6245888584980785No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGACTTGACTCTCA17520.6065851885191981No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA15510.5369940795623723No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTGACCAGCA15060.5214139805421875No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCCGGATGCTGA14220.4923311290378423No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA9470.32787452826922414No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGTTGATAGTCA9400.3254509573105287No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGCAATCACAGAAAG8750.3029463698369283No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTAAGATCCACCTTTA8750.3029463698369283No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGAATATTTGGTATGACCGAG7480.2589758681577398No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA7050.2440882179828965No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA6960.24097219817885954No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTGACCTGAA6840.2368175051068102No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA6170.21362046878786828No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA6130.2122355710971852No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATA5990.20738842917979433No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGTCCCGTACTCTG5590.19353945227296335No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA5550.19215455458228023No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTATGACCACCA5360.18557629055153552No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGATATTTGCAATCACAGAAAG4930.17068864037669218No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCCGGATATATGGGTGTACAGCCA4870.16861129384066753No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA4430.1533774192431534No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA4410.15268497039781187No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGAACAATAA4370.15130007270712875No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACTGATA4300.14887650174843334No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA4250.14714537963507945No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTCAAGTCCTGGGGCT3960.13710487137762697No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATCCCGTACTCTGCG3740.1294879340788699No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA3650.12637191427483294No Hit
CAGCTGTACACGGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC3640.12602568985216217Illumina Paired End PCR Primer 2 (100% over 36bp)
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTTATAATTA3540.1225634456254544No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA3000.10386732680123256No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)