FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002466624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583361
Sequences flagged as poor quality0
Sequence length289
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA7979913.679179787472936No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTAACAGAGA7753913.291769590356573No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA6731311.53882415862562No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATCACAGAAA365016.2570175243117045No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTCACCACCA257584.415447724479353No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA226403.8809587888117307No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATGGACAGTCA209893.5979436403873417No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACTGATA117342.0114474570634653No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA80991.3883341532944438No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA71191.2203421209165508No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA68521.1745728631156351No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA67421.1557166145834226No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA60551.0379507714776957No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA59101.0130948075034154No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA57610.9875531617643276No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA55320.9482978807290854No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGTTGATAGTCA52970.9080140770466314No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGAACAATAA47420.8128757321795594No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACTGTCA46890.8037904487958571No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA45330.7770488599683558No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATGACCGTCA44950.7705348832026824No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGATTCACGGTTA44250.7585354523185471No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCACCA41680.7144803989296508No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA41500.711394830988016No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA37250.638541143477195No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACATTGA30370.5206038799302661No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAGACAGTCA29990.5140899031645928No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA28270.48460558727786057No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTGACCATTA26650.45683547580314765No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA26490.45409274874391675No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAA25960.4450074653602144No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA25670.44003627256535827No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTATGACCACCA25520.4374649659473293No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCCACTTTTA25000.4285511030048289No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTCACAGTGA24040.4120947406494435No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA23680.4059236047661739No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA23600.4045522412365585No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTGTGACCAACA22480.3853531518219422No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGTTTTACTTGTA22030.3776392319678552No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA21080.3613542900536717No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGGATGATTA20640.35381179064078677No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTTTCACAGATA18660.3198705432828043No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTGACCAGAA16970.29090048871967783No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTGACCAGCA16770.2874720798956392No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACCACAA15800.27084429709905183No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCCGGATGCTGA15670.26861583136342676No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA15530.26621594518659975No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAAACCTTCA15340.262958956803763No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTAACAAATA15280.26193043415655143No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCAGCACGGACA13790.2363887884174636No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAACAGTCA13630.2336460613582327No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA13350.22884628900457865No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTATGACCGAGA13000.22284657356251106No Hit
CAGCTGGTACACGGCAGGGTCAAGGTTCTGGATATTTGGTATGACCGAGA12560.21530407414962605No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTTATAATTA12510.2144469719436164No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTTACTGTCA11730.2010761775298657No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACAGTTA11630.1993619731178464No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGTGGGACAAGAGG11260.19301941679337495No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA11250.192847996352173No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTGACCTGAA10990.18839106488092278No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA10870.18633401958649962No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGTTGTTGCCAGGGA10830.18564833782169188No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTTCTCCTCCAAAC10160.1741631682611625No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAAAGGAGTCAGGCTC9600.16456362355385432No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATGACTTGGA9340.16010669208260409No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAAAAAAGATAAGGG8850.15170709046370942No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTTATAATTA8470.14519311369803603No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA8440.14467885237443023No Hit
CAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC8250.14142186399159354Illumina Paired End PCR Primer 2 (100% over 48bp)
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGG8000.13713635296154525No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA7550.12942243310745835No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGCACTTGGA6710.11502311604649608No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGGGA6540.11210896854606325No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATGATGGTGA5990.10268084427995702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)