FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002466626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302114
Sequences flagged as poor quality0
Sequence length289
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA5585418.48772317734365No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA245338.120444600382637No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA198746.578311498308585No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA155715.154014709679128No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA119333.9498335065571273No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA119083.9415584845455687No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA109373.6201566296166345No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA82032.7152002224325917No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA78932.6125899494892657No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACCACAA77202.5553267971692804No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA67502.2342559431208087No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA65422.1654077599846415No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA64842.1462097089178256No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA62072.054522465029757No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA59631.9737582501969455No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA55441.8350688812832243No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA55281.8297728671958267No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA47611.5758951918812103No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA46781.548422118802836No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA40111.3276445315344538No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATA33961.1240789900501136No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA32581.0784008685463105No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACAGTTA31131.0304057408792708No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA31111.029743739118346No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA24570.8132691632959743No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCACCA24320.8049941412844158No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA21260.7037078718629391No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA19890.6583607512395984No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA18760.6209576517473536No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAGAATATGG15660.5183473788040276No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA15550.5147063691189419No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGATA13820.45744321679895666No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAACACAGAGGTAAG13660.4521472027115592No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA12240.40514507768590663No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA11790.3902500380651013No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAAAAAAGATAAGGG11520.381313014292618No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACTGTGA10520.3482129262463838No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAAGTCCTGGGGCT9240.30584481354720405No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTGACTCTCA9170.3035278073839676No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA8890.29425978273102205No Hit
CAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA8390.27770973870790494Illumina Paired End PCR Primer 2 (100% over 44bp)
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGCTGCGTCCCCCG8150.2697657175768088No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA7990.2644697034894113No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGTCTCCTCCAAAC7800.2581806867606268No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA5680.18800850010261028No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA5180.17145845607949317No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTACTTGGCTGGACA5050.16715544463348272No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAGAGCACAGAAGT4820.15954242438284888No Hit
CAGCTGGTACACGGCAGGGTCGGTTCTGGATATTGAGTTCCACTTTTAGC4580.15159840325175267No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC4300.14233037859880707Illumina Paired End PCR Primer 2 (100% over 49bp)
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA4150.13736536539187194No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTCCATGGGCATGG4120.13637236275048492No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCATTCCTCAGAGTCC3840.12710433809753932No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA3780.12511833281476528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)