FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002466628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99430
Sequences flagged as poor quality0
Sequence length289
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA1307913.153977672734587No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA89128.963089610781454No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA72687.3096650910188075No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAA72617.302624962285025No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA65156.552348385799054No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA44054.43025243890174No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA40044.026953635723625No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA37263.7473599517248317No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA29412.95785980086493No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA26352.6501056019310067No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA22932.3061450266519157No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA16241.633309866237554No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA15701.5790003017198029No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA13941.4019913506989843No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACAGTTA12591.2662174394046064No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACCACAA12461.2531429146132957No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATA12171.223976667001911No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA12011.2078849441818365No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA11771.1837473599517248No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAATGTGAAGAGCA11511.1575983103691039No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA11381.1445237855777934No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAGAATATGG11161.1223976667001911No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAAAAAAGATAAGGG10971.1032887458513527No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA9780.9836065573770493No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA9510.9564517751181736No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA8540.8588957055214724No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA7850.7895001508599014No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACTAGCA7590.7633511012772806No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA7390.7432364477521874No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA7350.7392135170471689No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA7020.7060243387307654No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA6560.6597606356230513No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA6350.6386402494217037No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA5940.597405209695263No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA5530.5561701699688223No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACTGTGA5250.5280096550336921No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA5000.5028663381273257No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA4460.4485567736095745No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA4290.43145931811324545No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA3600.36206376345167457No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTGACTCTCA3050.3067484662576687No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAAAGCACC2740.2755707532937745No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTCTAACACTCA2400.24137584230111636No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA2180.21924972342351404No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAGGGTCAGGAACA2130.21422106004224079No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA2050.20617519863220354No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGAATGGAG1940.1951121391934024No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGAATATCTGGTTTTACTTGTA1860.18706627778336518No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA1690.1699688222870361No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAGATTAACA1680.16896308961078146No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGGTGACTTGGA1520.15287136679070704No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGAATATCAGGTAAAACAGTCA1470.14784270340943376No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCACCA1390.13979684199939657No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTCATTTGGGCTTCA1210.12169365382681283No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA1210.12169365382681283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)