FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002466638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572537
Sequences flagged as poor quality0
Sequence length289
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTC13006022.71643579366923No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCCGC10648718.599147304017034No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCCTG9960317.39677959677715No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGG7242012.648964171747853No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCCGG234114.088993375100649No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAA152622.665679248677378No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGA147472.575728730195603No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTC106931.8676522216031455No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTG93281.6292396823262076No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC57691.0076204681968153No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGT52880.9236084305468467No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTG31780.5550732965729726No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCTGG30910.5398777720915854No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCCTA27920.48765407301187524No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGT25790.4504512372126168No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCCTG20980.36643919956264837No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCCTG20200.35281562588968046No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATG15870.2771873258846153No Hit
CGACCTCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC11680.20400428269264692Illumina Paired End PCR Primer 2 (100% over 45bp)
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC10740.18758612980471132Illumina Paired End PCR Primer 2 (100% over 49bp)
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGG7490.13082123950067856No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCCAGCACTGAGAGCCGGGT7480.13064657829974308No Hit
CGACCTCGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCA6650.11614969862209779No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT6600.11527639261742036Illumina Paired End PCR Primer 2 (100% over 50bp)
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGCCC6300.1100365565893558No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTACTCGAGCACTGTCAGCCGG6150.10741663857532352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)