FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002466639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572537
Sequences flagged as poor quality0
Sequence length289
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAACATCCTGAGGACAGTGC206683.609897700934612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGAATGCTTACTACAGAGACAC187663.2776920967553194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGCTTGGAGAGGGGTGGGTACT151732.65013440179412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAGTGACACTGATCTGGTAAA149952.6190447080276034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGAGGGGTGGGTACTGGAGAA147922.5835884842377Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGATACGCAGTATTTTGGCCCA146052.5509268396627642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGTATTTTGGCCCAGGCACCCG131032.288585715857665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGAGAGGGGTGGGTACTGGAGA124752.1788984816701804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCCTGAGGACAGTGCCTGGAG122882.1462368370952447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGCCCCAAGCTAGGAGATCCTG117552.053142416996631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAACATCCTGAGGACAGTGCC82791.4460200825448835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGCTTGGAGAGGGGTGGGTAC73331.2807905864599143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAAAAACATCCTGAGGACAGTG72831.27205752641314Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTTCACAGTGACACTGATCTGG64321.123420844417042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGATCCTGCCATGGGCTTCAGG60751.0610667956830737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGATCCTGCCATGGGCTTCAG59771.0439499979913962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACCAGCCCCAAGCTAGGAGATC59641.0416794023792348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACTGATCTGGTAAAGCCCCCAT58641.0242132822856864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCCTGGCCTGACCCTGCCATG58341.0189734462576217Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAACATCCTGAGGACAGTGCCT54420.9505062554909115Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATCTGGTAAAGCCCCCATCCT52790.9220364797384273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACTACAGAGACACCAGCCCCAA50130.8755766002895883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATCCTGAGGACAGTGCCTGGA46760.8167157755743297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGG46700.8156678083687167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATTTTGGCCCAGGCACCCGGCT43710.7634441092890066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGGGTGGGTACTGGAG42780.7472006176020065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGGAGATCCTGCCATGGGCTTC42730.7463273115973291Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAAACATCCTGAGGACAGTG39410.6883397928867478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGATACGCAGTATTTTGGCCC39200.6846719076671027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTTGGAGAGGGGTGGGTACTGGA36180.6319242249845861Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCTAGGAGATCCTGCCATGG35170.6142834436901021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAGATACGCAGTATTTTGGCC34650.6052010612414569Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGAGACACCAGCCCCAAGCTAG32690.5709674658581018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGCCCCAAGCTAGGAGATCCT28740.501976291488585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAAAACATCCTGAGGACAGTGC28410.49621247185771394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACAGTGACACTGATCTGGTAA28360.49533916585303656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATCCTGCCATGGGCTTCAGGC23560.4115017894040036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAAAAAACATCCTGAGGACAGT21940.38320667485245496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAGTGACCCTGATCTGGTAAA21700.3790148060300033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGAATGCTTACTACAGAGACA21660.3783161612262614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACAGATACGCAGTATTTTGGC20910.36521657115609996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTATTTTGGCCCAGGCACCCGG20190.352640964688745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTAAACATCCTGAGGACAGTGCC19090.33342823258584164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGTATTTTGGCCCAGGCACCC18670.32609246214655124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGCCTTACAAAGACATCCAGCT17970.31386617808106726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACATCCTGAGGACAGTGCCTGG17140.29936929840342197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTGGAGAGGGGTGGGTACTGGAG15390.268803588239712Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAGACATCCAGCTCTAAGGAG14550.25413204736113126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAGCCCCCATCCTGGCCTGA14550.25413204736113126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGAGGGGTGGGTACTGGAGAAG14450.2523854353517764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGAGATCCTGCCATGGGCTTCA14380.251162806945228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACCACAGATACGCAGTATTTTG14100.2462722933190344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAGCCCCCATCCTGGCCTGAC14090.2460976321180989Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACTCTGAGAGGGGCTGTGCTGA13660.23858720047787307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCCTCTGCTTCTGTTCACAGT13400.23404600925355043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTCACAGTGACACTGATCTGGTA13350.23317270324887301Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGACACCAGCCCCAAGCTAGGA13300.23229939724419557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACCAGCCCCAAGCTAGGAGAT13150.22967947923016327Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAACACCATATATTTTGGAGA13130.2293301568282923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGCAGTATTTTGGCCCAGGCACC12990.2268849000151955Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAACAGCCTTACAAAGACATCC12770.22304235359461486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACACCAGCCCCAAGCTAGGAG12650.22094641918338903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCTGGTAAAGCCCCCATCCTG12600.2200731131787116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAAGCTAGGAGATCCTGCCATG12420.21692921156187286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACTCATGATGCACTGTGTAGCA12340.21553192195438897Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC12170.2125626815384857Illumina Single End PCR Primer 1 (100% over 49bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTAAAACATCCTGAGGACAGTGC11370.19858978546364686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACACAAGATCCTGCCCTGGAG11290.19719249585616302Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATCCTGGCCTGACCCTGCCAT11280.19701783465522751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAACAGCCTTACAAAGACATC11280.19701783465522751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAGAGACACCAGCCCCAAGCT11230.19614452865055007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTTCTGTTCACAGTGACACTGAT11230.19614452865055007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGGAATTTGAAGTAGATAGAA11160.1949219002440017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGATCTGGTAAAGCCCCCATCC11090.1936992718374533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAAAACATCCTGAGGACAGT11020.1924766434309049Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGACTACAGAGACACCAGCCCCA10880.1900313866178081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAAGATCCTGCCCTGGAGCTG10620.1854901953934855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAACATCCTGAGGACAGTGCCTG10580.18479155058974353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTATCATGGTGTAACATTGTGG10570.18461688938880805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATTTTGGCCCAGGCACCCGGC10440.18234629377664674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGCTTCTGTTCACAGTGACACTG10180.17780510255232415Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGGTTCACAGTGACACTGATCTG9960.17396255613174344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACAAAGACATCCAGCTCTAAGG9630.16819873650087241Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGCGGGAGAGGGGTGGGTACTGGA9570.16715076929525952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTTCACAGTGACACTGATCTGGT9440.1648801736830982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGTTTCTGTGCCGCGTCTCGGG9280.16208559446813042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAACACCATATATTTTGGAGAG9220.16103762726251755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGCATTTTGGTGATGGGACTCG9090.15876703165035622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTTGGAGAGGGGTGGGTACTGG9030.15771906444474332Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTGGGAGAGGGGTGGGTACTGGA8890.15527380763164653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAACAAACACCATATATTTTGGA8560.1495099880007755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTAAAACATCCTGAGGACAGTG8430.1472393923886142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAGACATCCAGCTCTAAGGA7980.13937963834651734Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACACAGATACGCAGTATTTTGG7970.13920497714558186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTAAACATCCTGAGGACAGTGC7920.13833167114090444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGGAGATCCTGCCATGGGCTT7900.13798234873903345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCCTGCCATGGGCTTCAGGCT7900.13798234873903345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGACACAGTGACACTGATCTGGTA7810.1364103979306141Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGAGATGAGATCCTGGCCTGG7740.1351877695240657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7620.13309183511283987Illumina Single End PCR Primer 1 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATGCTTGTAAGCTCCTTCATCT7580.13239319030909794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGAGGGGCTGTGCTGAGAGGC7250.1266293706782269Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGAGGCCCCATCTCAGACCCGA7010.12243750185577526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGAGGTAAGCAGCTGCCCCACT6960.12156419585109784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGTGTTGGAGAGGGGTGGGTACTG6860.11981758384174297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTAGAAGCCTGTGGCCAGGCAC6740.11772164943051715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAAACATCCTGAGGACAGTGCC6730.11754698822958166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTTCCTGAGGACAGTGCCTGGA6720.11737232702864618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATGTATCCCTTTTTTGGTACCA6710.1171976658277107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGCACAGCCTCGGGGCAGGGCCA6640.1159750374211623Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATATATTTTGGAGAGGGAAGTT6550.11440308661274294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATCCCTTTTTTGGTACCAACAG6330.11056054019216224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAGGAGGACCAAGCCCTGAATCA6310.11021121779029128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATAAGGACATGCCCCAACGTGA6210.10846460578093643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAAAGCCAAGCAGGGATATCTG6160.107591299776259Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGATTGTGGGGACTAGCCCAATT6070.10601934896783964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGTGCTGAGAGGCGCTGCTGGGC5950.10392341455661382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGATGCCCCAACGTGACAACAGCT5910.10322476975287186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGGGGAGTGACGCTGAGATGCTCCCC5900.10305010855193639Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph